Effect of lead stress on polyphenols, flavonoids, and proline contents in radish (Raphanus sativus L.)

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Research Paper 01/05/2018
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Effect of lead stress on polyphenols, flavonoids, and proline contents in radish (Raphanus sativus L.)

Nour Elhouda Neggaz, Houcine Abdelhakim Reguieg Yssaad
Int. J. Biosci.12( 5), 133-142, May 2018.
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Abstract

Radish (Raphanus sativus L.) is a suitable modal plant for eco-toxicological studies due to its tolerance to various stresses, such as metallic and saline stress. Lead is a toxic and exogenous metal to plants, and its presence causes several disturbances in plant. This work consists to determine the effect of lead stress applied during two weeks (0, 500, 1000, 2000 ppm) after 45 days of the plant’s growth. The parameters analyzed in the plant are polyphenols, flavonoids, and proline. The assay is performed using a JENWAY 6505 UV-Visible Spectrophotometer. The results obtained show a decrease in proline, but polyphenols and flavonoids show a marked increase. The high levels of proline (0.117 mg/g dry matter in the control) and polyphenols (0.248 mg/g dry matter at the 2000 ppm dose of lead) are recorded in the root system. However, the highest content of flavonoid (8,586 mg/g dry matter in the control) is recorded in the aerial part of the plant. Radish (Raphanus sativus L.) is a tolerant plant to lead stress and has a phytoremediator power for lead.

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