Green antibacterial approach: Ultrasound-enhanced date seed (Phoenix dactylifera L.) extracts against Escherichia coli
Paper Details
Green antibacterial approach: Ultrasound-enhanced date seed (Phoenix dactylifera L.) extracts against Escherichia coli
Abstract
Natural Deep Eutectic Solvents (NADES) are novel greener and sustainable solvents that enhance phenolic compound extraction from different plant sources. The study aims to extract date seeds using three natural solvents and ultrasound and test the antibacterial effect of date seeds extracts against Escherichia coli bacteria by using “the well diffusion” method to measure the zone of inhibition (ZOI). The results show that the date seeds extracted with NADES such as; lactic and citric acids significantly exhibited the highest ZOI values (40.5 ± 0.71 and 36.0 ± 2.83 mm, respectively) (p < 0.05) compared to the standard antibiotic Ciprofloxacin (29.5 ± 0.71 mm) and other date seeds extracts. Also, it found that each date seeds extract significantly exhibited (p < 0.05) higher ZOI values than its extracted solvent. The study recommends that the extraction process enhance the antibacterial properties of the extracts. These findings noted that NADES containing carboxylic acids have the potential to extract polyphenols from date seeds with notable antibacterial activities. NADES can be utilized in various industries such as food and pharmaceuticals as environmentally friendly solvents.
Alkhoori MA, Kong ASY, Aljaafari MN, Abushelaibi A, Erin Lim SH, Cheng WH, Chong CM, Lai KS. 2022. Biochemical Composition and Biological Activities of Date Palm (Phoenix dactylifera L.) Seeds: A Review. Biomolecules 12(11), 1626.
Bakirtzi C, Triantafyllidou K, Makris DP. 2016. Novel lactic acid-based natural deep eutectic solvents: Efficiency in the ultrasound-assisted extraction of antioxidant polyphenols from common native Greek medicinal plants. Journal of Applied Research on Medicinal and Aromatic Plants 3(3), 120-127.
Barakat AZ, Hamed AR, Bassuiny RI, Abdel-Aty AM, Mohamed SA. 2020. Date palm and saw palmetto seeds functional properties: antioxidant, anti-inflammatory and antimicrobial activities. Journal of Food Measurement and Characterization 14(2), 1064-1072.
Ben-Youssef S, Fakhfakh J, Breil C, Abert-Vian M, Chemat F, Allouche N. 2017. Green extraction procedures of lipids from Tunisian date palm seeds. Industrial Crops and Products 108, 520-525.
CLSI, Clinical and Laboratory Standards Institute. 2016. Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Susceptibility Testing, CLSI Supplement M100S (26th ed). Clinical and Laboratory Standards Institute.
Dai Y, van Spronsen J, Witkamp GJ, Verpoorte R, Choi YH. 2013. Natural deep eutectic solvents as new potential media for green technology. Analytica Chimica Acta 766, 61-68.
Dheyab AS, Abu Bakar MF, Alomar M, Sabran SF, Muhamad Hanaf AF, Mohamad A. 2021. Deep Eutectic Solvents (DESs) as Green Extraction Media of Beneficial Bioactive Phytochemicals. Separations 8(10), 176.
Fuad FM, Nadzir MM, Harun A. 2021. Hydrophilic natural deep eutectic solvent: A review on physicochemical properties and extractability of bioactive compounds. Journal of Molecular Liquids 339, 116923.
Grozdanova T, Trusheva B, Alipieva K, Popova M, Dimitrova L, Najdenski H, Zaharieva MM, Ilieva Y, Vasileva B, Miloshev G, Georgieva M, Bankova V. 2020. Extracts of medicinal plants with natural deep eutectic solvents: enhanced antimicrobial activity and low genotoxicity. BMC Chemistry 14(1), 1-9.
Ivanović M, Grujić D, Cerar J, Razboršek MI, Topalić-Trivunović L, Savić A, Kočar D, Kolar M. 2022. Extraction of Bioactive Metabolites from Achillea millefolium L. with Choline Chloride Based Natural Deep Eutectic Solvents: A Study of the Antioxidant and Antimicrobial Activity. Antioxidants 11(4), 724.
Jahangirian H, Haron MJ, Shah Ismail MH, Rafiee-Moghaddam R, Afsah-Hejri L, Abdollahi Y, Rezayi M, Vafaei N. 2013. Well diffusion method for evaluation of antibacterial activity of copper phenyl fatty hydroxamate synthesized from canola and palm kernel oils. Digest Journal of Nanomaterials and Biostructures 8(3), 1263-1270.
Juneidi I, Hayyan M, Mohd Ali O. 2016. Toxicity profile of choline chloride-based deep eutectic solvents for fungi and Cyprinus carpio fish. Environmental Science and Pollution Research 23(8), 7648-7659.
Jurić T, Mićić N, Potkonjak A, Milanov D, Dodić J, Trivunović Z, Popović BM. 2021. The evaluation of phenolic content, in vitro antioxidant and antibacterial activity of Mentha piperita extracts obtained by natural deep eutectic solvents. Food Chemistry 362, 130226.
Karadendrou MA, Kostopoulou I, Kakokefalou V, Tzani A, Detsi A. 2022. L-Proline-Based Natural Deep Eutectic Solvents as Efficient Solvents and Catalysts for the Ultrasound-Assisted Synthesis of Aurones via Knoevenagel Condensation. Catalysts 12(3), 249.
Li D. 2022. Natural deep eutectic solvents in phytonutrient extraction and other applications. Frontiers in Plant Science 13, 1004332.
Madigan MT, Martinko JM, Dunlap PV, Clark DP. 2008. Brock biology of microorganisms 12th edn. International Microbiology 11, 65-73.
Makarewicz M, Drożdż I, Tarko T, Duda-Chodak A. 2021. The interactions between polyphenols and microorganisms, especially gut microbiota. Antioxidants 10(2), 1-70.
Maqsood S, Adiamo O, Ahmad M, Mudgil P. 2020. Bioactive compounds from date fruit and seed as potential nutraceutical and functional food ingredients. Food Chemistry 308, 125522.
Metoui M, Essid A, Bouzoumita A, Ferchichi A. 2019. Chemical composition, antioxidant and antibacterial activity of Tunisian date palm seed. Polish Journal of Environmental Studies 28(1), 267-274.
Papuc C, Goran GV, Predescu CN, Nicorescu V, Stefan G. 2017. Plant Polyphenols as Antioxidant and Antibacterial Agents for Shelf-Life Extension of Meat and Meat Products: Classification, Structures, Sources, and Action Mechanisms. Comprehensive Reviews in Food Science and Food Safety 16(6), 1243-1268.
Pavić V, Flačer D, Jakovljević M, Molnar M, Jokić S. 2019. Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents. Plants 8(3), 69.
Platat C, Habib HM, AL Maqbali FD, Jabar NN, Ibrahim WH. 2014. Identification of Date Seeds Varieties Patterns to Optimize Nutritional Benefits of Date Seeds. Journal of Nutrition & Food Sciences S8(2).
Radošević K, Čanak I, Panić M, Markov K, Bubalo MC, Frece J, Srček VG, Redovniković IR. 2018. Antimicrobial, cytotoxic and antioxidative evaluation of natural deep eutectic solvents. Environmental Science and Pollution Research 25(14), 14188-14196.
Silva SNRL, Farias CBB, Rufino RD, Luna JM, Sarubbo LA. 2010. Glycerol as substrate for the production of biosurfactant by Pseudomonas aeruginosa UCP0992. Colloids and Surfaces B: Biointerfaces 79(1), 174-183.
Xin R, Qi S, Zeng C, Khan FI, Yang B, Wang Y. 2017. A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties. Food Chemistry 217, 560-567.
Zannou O, Koca I. 2022. Greener extraction of anthocyanins and antioxidant activity from blackberry (Rubus spp) using natural deep eutectic solvents. Lwt 158, 113184.
Zhao BY, Xu P, Yang FX, Wu H, Zong MH, Lou WY. 2015. Biocompatible Deep Eutectic Solvents Based on Choline Chloride: Characterization and Application to the Extraction of Rutin from Sophora japonica. ACS Sustainable Chemistry and Engineering 3(11), 2746-2755.
Alanood A. Alfaleh, Marya J. Hamdan, Abdullatif A. Neamatallah, Nagwa T. Elsharawy, 2024. Green antibacterial approach: Ultrasound-enhanced date seed (Phoenix dactylifera L.) extracts against Escherichia coli. Int. J. Biosci., 25(1), 138-146.
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