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In-vitro Cytotoxicity of Wrightia pubescens (Blanco) Merr., Aphanamixis polystachya (Wall.) Parker, and Platymitra arborea (Blanco) against selected human cancer cell lines

By: Maria Lorraine Garcia Bugayong, Sonia Donaldo Jacinto

Key Words: Cytotoxicity, Anticancer, Plant extracts, Hepatocellular carcinoma, Colorectal cancer

Int. J. Biosci. 11(5), 204-213, November 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.5.204-213

Certification: ijb 2017 0146 [Generate Certificate]

Abstract

Cancer is the second leading cause of mortality worldwide. Anticancer studies are centered on natural products as these have been found to exhibit properties that activate cell signaling pathways and cell aging and senescence. As such, there is a need for continuing research to explore these natural products especially those that do not only exhibit cytotoxicity but also specificity against cancer cells. This study evaluated the cytotoxic activity of the crude extracts of three Philippine indigenous plants, Wrightia pubescens (Blanco) Merr. Aphanamixis polystachya (Wall.) Parker, and Platymitra arborea (Blanco) against selected human cancer cell lines using 3-(4,5- dimethylthiazol-2-yl)-2-5-diphenyl-2H-tetrazolium bromide (MTT) assay. Extracts were partitioned using hexane and ethyl acetate to determine their active fractions based on their polarity. These active fractions were then tested for their cytotoxicity against human colorectal cancer cell line (HCT116), human adenocarcinoma cell line (A549) and non-cancer Chinese hamster ovary cell line (AA8). Cytotoxic activities of the extracts were found in the ethyl acetate fractions of W. pubescens and P. arborea and hexane fraction of A. polystachya. All active fractions were highly cytotoxic to HCT116 and A549, with A. polystachya exhibiting the highest selectivity against cancer over the non-cancer cells. Results of this study imply that these extracts, especially that of A. polystachya, have a potential use in anti-cancer research due to their selectivity against cancer cell lines.

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In-vitro Cytotoxicity of Wrightia pubescens (Blanco) Merr., Aphanamixis polystachya (Wall.) Parker, and Platymitra arborea (Blanco) against selected human cancer cell lines

Abdul A, Abdel-Wahab S, Fong HK, Mohan SM, Al-Zubair AS, Elhassan MM. 2009. In vitro Response of Cancer Cells to the Growth-Inhibitory Effects of Dichloromethane Extract of Goniothalamus umbrosus. Research Journal of Pharmacology 3, 1-6. www.medwelljournals.com/abstract/?doi=rjpharm.2009.1.6

American Type Culture Collection. 1999. www.atcc.org/

Brown T, Pilkington G, Bagust A, Boland A, Oyee J, Tudur-Smith C, Blundell M, Lai M, Martin Saborido C, Greenhalgh J Dundar Y, Dickson R. 2013. Clinical effectiveness and cost-effectiveness of first-line chemotherapy for adult patients with locally advanced or metastatic non-small cell lung cancer: a systematic review and economic evaluation. Health Technology Assessment 17, 1-278.

http://dx.doi.org/10.3310/hta17310

Chan L, George S, Ahmad I, Gosangari S, Abbasi A, Cunningham B, Watkin K. 2011. Cytotoxicity Effects of Amoora rohituka and chittangonga on Breast and Pancreatic Cancer Cells. Evidence based Complementary and Alternative Medicine. Epub 2011 Apr 26.

http://dx.doi.org/10.1155/2011/860605

Chakravarti B, Maurya R, Siddiqui JA, Bid HK, Rajendran SM, Yadav PP, Konwar R. 2012. In vitro anti-breast cancer activity of ethanolic extract of Wrightia tomentosa: role of proapoptotic effects of oleanolic acid and urosolic acid. Journal of Ethnopharmacology 142, 72-79.

http://dx.doi.org/10.1016/j.jep.2012.04.015

Cragg GM, Newman DJ. 2005. Plants as a Source of Anti-cancer agents. Journal of Ethnopharmacology 100, 72-29.

http://doi.org/10.1016/j.jep.2005.05.011

Graham JG, Quinn ML, Fabricant DS, Farnsworth NR. 2000. Plants used against cancer- an extension of the work of Jonathan Hartwell. Journal of Ethnopharmacology 73, 47-377.

http://dx.doi.org/10.1016/S0378-8741(00)00341-X

Harmon AD, Weiss U, Silverton JV. 1979. The structure of rohitukine, the main alkaloid of Amoora rohituka (syn. Aphanamixis polystachya) (Meliaceae. Tetrahedron Letters 8, 721-724.

Jagetia G, Venkatesha V. 2006. Treatment of mice with stem bark extract of Aphanamixis polystachya reduces radiation-induced chromosome damage. International Journal of Radiation Biology 82, 197-209.

http://dx.doi.org/10.1080/09553000600621413

Jagetia GC, Venkatesha VA. 2005. Enhancement of radiation effect by Aphanamixis ploystachya in mice transplanted with Ehrlich ascites carcinoma. Biological & Pharmaceutical bulletin  28, 69-77.

http://dx.doi.org/10.1248/bpb.28.69

Jagetia GC, Venkatesha VA. 2016. Determination of Antineoplastic activity of Rohituka, Aphanamixis polystachya (Wall) RN Parker in Hela Cells: Correlation with Clonogenicity and DNA Damage. International Journal of Complementary & Alternative Medicine  3, 1-11.

http://dx.doi.org/10.15406/ijcam.2016.03.00083

Jain P, Bari S. 2009. Antibacterial and Antifungal activity of extracts of woody stem of Wrightia tinctoria R. Br. International Journal of Pharmaceutical Sciences and Research 1, 18-21.

Jokhadze M, Eristavi L, Kutchukhidze J, Chariot A, Angenot L, Tits M, Jansen O, Frederich M. 2007. In vitro cytotoxicity of some medicinal plants from Georgian Amaryllidaceae. Phytotheraphy Research 21, 622-624.

www.dx.doi.org/10.1248/bpb.28.6910.1248/bpb.28.69

Kannan P, Shanmugavadivu B, Petchiammal C, Hopper W. 2006. In vitro antimicrobial activity of Wrightia tinctoria leaf extracts against skin microorganisms. Acta Botanica Hungarica 48, 323-329.

http://dx.doi.org/10.1556/ABot.48.2006.3-4.7

Kasper D, Fauci A, Hauser S, Longo D, Jameson J. 2015. Cancer genetics. In Kasper,DL Ed. Harrison’s principles of internal medicine. New York. McGraw-Hill, Medical Pub. Division. 102e-1- 102e9 P.

Krishnaraju A, Rao C, Rao T, Reddy KN, Trimurtulu G. 2009. In vitro and In vivo Antioxidant Activity of Aphanamixis polystachya Bark. American Journal of Infectious Diseases 5, 60-67.

Lin L, Topcu G, Lotter H, Ruangrungsi N, Wagner N, Pezzuto J, Cordell G. 1992. Wrightiadone from Wrightia tomentosa. Phytochemistry 31, 4333-4335.

http://dx.doi.org/10.1248/bpb.28.69

Lopez-Lazaro, M. 2015. Two precliicial tests to evaluate anticancer activity and to help validate drug candidates for clinical trials. Oncoscience 2, 91-98.  http://dx.doi.org/10.18632/oncoscience.132

Machana S, Weerapreeyakul N, Barusrux S, Nonpunya A, Sripanidkulchai B, Thitimetharoch T. 2011. Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line. International Society for Chinese Medicine 6, 39.

http://dx.doi.org/10.1186/1749-8546-6-39

Middleton DJ. 2005. A Revision of Wrightia (Apocynaceae: Aposcynoideae) in Malesia. Harvard Papers in Botany 10, 161-182.

Mijatovic T, Mathieu V, Gaussin J-F, De Neve N, Ribaucour F, Van Quaquebeke E, Dumont P, Darro F, Kiss R. 2006. Cardenolide-induced lysosomal membrane Permeabilization demonstrates therapeutic benefits in Experimental Human Non-small cell lung cancers. Neoplasia 8, 402-412.

http://dx.doi.org/10.1593/neo.05850

Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immulogical methods 65, 55-63.

Mulholland DA, Naidoo N. 1999. Limonoids from Aphanamixis polystachya. Phytochemistry  51, 927-930.

 http://dx.doi.org/10.1016/S0031-9422(99)001570

Oliveira PFd, Alves JM, Damasceno JL, Oliveira RAM, Júnior HD, Crotti AEM, Tavares DC. 2015. Cytotoxicity screening of essential oils in cancer cell lines. Revista Brasileira de Farmacognosia 25, 183-188.

http://dx.doi.org/10.1016/j.bjp.2015.02.009

Pan L, Chai HB, Kinghorn AD. 2012. Discovery of new anticancer agents from higher plants. Frontiers in Bioscience 4, 142-156.

http://dx.doi.org/10.2741/257

Pradupsri P, Loetchutinat C, Nuntasaen N, Meepowpan P, Tuntiwechapikul W, Pompimon W. 2009. Anticancer Activities of Styrylpyrone from the Leaves and twigs of Goniothalamus maewongensis via cell cycle arrest. American Journal of Applied Sciences 6, 2018.

http://dx.doi.org/10.3844/ajassp.2009.2018.2023

Prayong P, Barusrux S, Weerapreeyakul N. 2008. Cytotoxic activity screening of some indigenous Thai plants. Fitoterapia 79, 598-601.

Jagetia GC, Venkatesha VA. 2016. Determination http://dx.doi.org/10.1016/j.fitote.2008.06.007

Ragasa C, Ng VA, De Los Reyes M, Mandia E, Shen CC. 2014a. Chemical constituents of Wrightia pubescens (R.Br.). Der Pharmacia Lettre 6, 14-19.

Ragasa C, Ng VA, De Los Reyes M, Mandia E, Shen CC. 2015. An Isoflavone from Wrightia pubescens. International Journal of Pharmacognosy and Phytochemical Research 7, 353-355.

Ragasa C, Aguilar MLT, Ng VA, Bugayong ML, Jacinto SD, Wen-Tai L, Shen CC. 2014b. Terpenoids and sterol from Aphanamixis polystachya. Journal of Chemical and Pharmaceutical Research 6, 65-68.

Rahmatullah M, Rahman L, Rehana F, Azad AK. 2010. A scientific evaluation of Medicinal plants used in the Folk Medicinal System of Five villages in Narsinghdi District, Bangladesh. American-Eurasian Journal of Sustainable Agriculture 4, 55-64.

Schilling D, Duwel M, Molls M, Multhoff G. 2013. Radio sensitization of wild type p53 cancer cells by the MDM2-inhibitor PXN727 is associated with altered heat shock protein 70 (Hsp70) levels. Cell Stress Chaperones 18, 183-191.

http://dx.doi.org/10.1007/s12192-012-0369-8

Seigel R, Miller K, Jemal A. 2017. Cancer Statistics, 2017. CA: A Cancer Journal for Clinicians

67, 7-30.

http://dx.doi.org/10.3322/caac.21387

Selvam P, Murugesh N, Witvrouw M, Keyaerts E, Neyts J. 2009. Studies of antiviral activity and cytotoxicity of Wrightia tinctoria and Morinda citrifolia. Indian Journal of Pharmaceutical Sciences 71, 670-672.

http://dx.doi.org/10.4103/0250-474X.59550

Suffness M, Pezzuto JM. 1990. Assays related to cancer drug discovery. In: Hostettmann, K, Ed. Methods in Plant Biochemistry, vol. 6. London: Academic Press. p 71-133.

World Health Organization. February 2017. Cancer Fact Sheet.

www.who.int/mediacentre/factsheets/fs297/en/

Yu D. 1999. Recent works on anti-tumor constituent from  Annonaceae plants in China. Pure and Applied Chemistry 71, 1119-1122.

http://dx.doi.org/10.1351/pac199971061119

Maria Lorraine Garcia Bugayong, Sonia Donaldo Jacinto.
In-vitro Cytotoxicity of Wrightia pubescens (Blanco) Merr., Aphanamixis polystachya (Wall.) Parker, and Platymitra arborea (Blanco) against selected human cancer cell lines.
Int. J. Biosci. 11(5), 204-213, November 2017.
https://innspub.net/ijb/vitro-cytotoxicity-wrightia-pubescens-blanco-merr-aphanamixis-polystachya-wall-parker-platymitra-arborea-blanco-selected-human-cancer-cell-lines/
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