Optimization of probiotic activity of Lactobacillus acidophilus characterized from commercial yoghurt
Paper Details
Optimization of probiotic activity of Lactobacillus acidophilus characterized from commercial yoghurt
Abstract
In this study, five (5) Lactobacillus acidophilusisolates characterized from local yoghurt sample after enrichment in MRS broth using biochemical and morphological examination. Two of the five, isolate LA002 and LA005, posed best probiotic activity on test organisms.Al of theL. acidophilusisolates grew best at 37°C with an initial pH 5. However, the best activity ofL. acidophilus was observed in pH 6. The two lactic acid bacterial isolates, LA002 and LA005 demonstrated potent antimicrobial activity against different multidrug resistant isolates of Escherichia coli, Klebsiella pneumonia and Salmonella typhimorium. The L. acidophilus was found to be the most potent antagonistic microbe against E. coli and activity was much higher than onion and garlic. K. pneumonia also significantly inhibited by L. acidophilusisolates. However, L. acidophilus isolates can not considerably inhibit the growth of S. typhimorium.These results demonstrated that L. acidophilus bacteria can support a healthy digestive tract and can be used as a probiotic supplementation.
Alla A, Aroutcheva J, Simoe A, Faro S. 2003. Antimicrobial protein produced by vaginal Lactobacillusacidophilus that inhibits Gardenella vaginalis. Parthenon Publishing9, 33-39. http://dx.doi.org/10.1155/S1064744901000060
Aslam S, Qazi JI. 2010. Isolation of acidophilic lactic acid bacteria antagonistic to microbial contaminants. Pakistan Journal of Zoology 42(5), 567-573.
Corzo G, Gilliland S. 1999. Bile salt hydrolase activity of three strains of Lactobacill acidophilus. Journal of Dairy Science 82, 472-480. http://dx.doi.org/10.3168/jds.S00220302(99)75256-2
Daeschel MA. 1989. Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food Technology 43, 164.
Foysal MJ, Rahman MM, Alam M. 2011. Antibiotic sensitivity and in-vitro antimicrobial activity of plant extracts to Pseudomonas fluorescens collected from diseased fish. International Journal of Natural Sciences 1, 82-88. http://dx.doi.org/10.3329/ijns.v1i4.9733
Foysal MJ, Rahman MM, Hossain MN, Yeasmin FM, Miyan SH. 2012. Antibiotic susceptibility profiling and in-vitro antibacterial activity of some plant extracts to Escherichia coli characterized from spoiled rice and egg. International Journal of Biosciences 2, 40-46.
Foysal MJ, Rahman MM, Prodhan SH. 2013. PCR based molecular detection of gyrB2 gene from Klebsiella sp. Isolates from patients who were suffering with pneumonia and urinary tract infections (UTI). Journal of Clinical and Diagnostic Research 7 ,23-25. http://dx.doi.org/10.7860/JCDR/2012/4661.2661
Fuller R. 1989. Probiotics in man and animals. Journal ofApplied Bacteriology 66,365-378.
Goderska K, Czarncki Z. 2007. Characterization of selected strains from Lactobacillus acidophilus and Bifidobacterium bifidum. African Journal of microbiology Research 1, 65-78.
Hassan P, Peh KK. 2014.Characterization and identification of lactobacillus acidophilus using biolog rapid identification system. International Journal of Pharmacy and Pharmaceutical Sciences 6, 189-193.
Hummel AS, Hertel C, Holzapfel WH, Franz C. 2007. Antibiotic resistance of starter and probiotic strains of Lactic acid bacteria. Applied and Environmental Microbiology 73, 730-739. http://dx.doi.org/10.1128/AEM.02105-06
Liong MT, Shah MT. 2005. Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. Journal of Dairy Science88, 55-66. http://dx.doi.org/10.3168/jds.S00220302(05)72662-X
Millette M, Luquet FM, Lacroix M. 2007.In vitro growth control of selected pathogens by Lactobacillus acidophilus and Lactobacillus casei-fermented milk. Letter in Applied Microbiology 44, 314-319. http://dx.doi.org/10.1111/j.1472-765X.2006.02060.x
Ng SC, Hart AL, Stagg AJ, Knight SC. 2009. Mechanisms of action of probiotics: recent advances. Inflammatory Bowel Diseases 15, 300-310. http://dx.doi.org/10.1002/ibd.20602
Parker B. 1974.Probiotics, the other half of the antibiotics story. Animal Nutrition and Health 29, 4-8.
Shah NP. 2007. Functional cultures and health benefits. International Dairy Journal 17(11), 1262-1277. http://dx.doi.org/10.1016/j.idairyj.2007.01.014
Usman HA. 1999. Bile tolerance, taurocholate deconjugation and binding of cholesterol by Lactobacillus gasseri strains. Journal of Dairy Science 82, 243-248. http://dx.doi.org/10.3168/jds.S00220302(99)75229-X
Zinedine A, Faid M. 2007. Isolation and characterization of strains of Bifidobacteria with probiotic properties in vitro.World Journal of Dairy and Food Sciences 2, 28-34.
Md. Ashrafuzzaman, Farhana Momtaz, Md. Javed Foysal, Md. Hazrat Ali, Md. Mahbubur Rahman, 2015. Optimization of probiotic activity of Lactobacillus acidophilus characterized from commercial yoghurt. Int. J. Biosci., 7(5), 94-99.
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