Phytochemical analysis, antibacterial and antioxidant activities of pomegranate (Punica granatum L.) peel extracts

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Research Paper 01/06/2020
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Phytochemical analysis, antibacterial and antioxidant activities of pomegranate (Punica granatum L.) peel extracts

Rachida Benguiar, Imene Yahla, Rachida Benaraba, Sarah Bouamar, Ali Riazi
Int. J. Biosci.16( 6), 35-44, June 2020.
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The pomegranate and their derivative parts are rich source of phytochemicals with multiple beneficial biological effects. This study aims to extract the phenolic compound from pomegranate (Punica granatum L.) peel using methanol and ethanol in order to assess their antioxidant and antibacterial activities. The phenolic compounds and flavonoids content were measured using colorimetric methods. The antioxidant activity was determined by ferric reducing antioxidant power (FRAP). Furthermore, the antibacterial activity of peel extracts was tested on Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram negative bacteria (Escherichia coli) using the agar diffusion and microbroth dilution methods. The results have shown that the pomegranate peel of both extracts contained a similar amount of phenolic compounds and flavonoids. However, the antioxidant activity was significantly (p<0.05) higher in ethanolic extract with EC50 value of 58.42± 0.21µg/mL compared to methanolic extract (EC50 = 80±1µg/mL). Both extracts exhibited a good antibacterial effect against Staphylococcus aureus and Bacillus subtilis with minimal inhibitory concentration (MIC) values ranging from 0.97 to 3.9 mg/mL and minimal bactericidal concentration (MBC) values ranging from 7.81 to 62.5 mg/mL. According to these findings, the pomegranate peel extracts have an important antibacterial and antioxidant properties and may be used as an alternative to antibiotics in the treatment of infections and in the prevention of pathologies associated with oxidative stress.


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