Production of herbal fermented honey using isolated Candida sp. and analysis of antimicrobial and antioxidant activity

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Research Paper 12/12/2023
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Production of herbal fermented honey using isolated Candida sp. and analysis of antimicrobial and antioxidant activity

S. Jamuna Devi, L. Prince
Int. J. Biosci.23( 6), 215-223, December 2023.
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Abstract

Honey use and production has a long and varied history. In many cultures, honey has associations that go beyond its use as a food. It is frequently used as a talisman and symbol of sweetness. For more than 5000 years, Indians have been continually using it, today there are lots of practitioners, and there are Academies founded all over the world. People trust it and ayurveda remains one of the most natural ways of health care. In the present study was carried production of herbal fermented honey using isolated Candida sp. and analysis of antimicrobial and antioxidant activity. The spoiled fruit samples were collected from Market at Pudukkottai district and isolate the Candida sp. The isolated Candida sp. was conformation by 18 S rRNA sequencing and fermentation was performed using isolated Candida sp. with garlic in carboy. Analysis of antimicrobial and antioxidant activity such as superoxide anion radical scavenging activity and reducing power activity was using herbal fermented honey. Fermented honey active compounds were analyzed by FT-IR. HiCrome Candida Differential Agar is a selective and differential medium, which facilitates rapid isolation of yeasts from mixed cultures and allows differentiation of Candida species namely C. albicans, C. krusei and C. glabrata on the basis of coloration and colony morphology. Based on the molecular characteristics and sequence alignments the isolated strain was conformed as C. albicans. FT-IR spectrum confirmed the presence of Alcohols, Phenols, Alkanes, Primary amines, Aromatics, Aliphatic amines and Nitro compounds.

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Bardy J, Slevin NJ, Mais KL, Molassiotis A. 2008. A systematic review of honey uses and its potential value within oncology care. Journal of clinical nursing 17(19), 2604–2623. https://doi.org/10.1111/j.1365-2702.2008.02304.x

Bauer AW, Kirby MM, Sherris JC, Truck M. 1996. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45, 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493

Bhadra B, Rao SR, Singh PK, Sarkar PK, Shivaji S. 2008. Yeasts and yeast-like fungi associated with the bark of trees: Diversity of yeasts and identification of yeasts producing extracellular endo-xylanases. Current Microbiology 56, 489-494. https://doi.org/ 10.1007/s00284-008-9108-x

Cappuccino GG, Sherman N. 1999. Microbiology a Laboratory Manual Rockland Aommunity College, Suffern  New York

Conboy L, Edshteyn I, Garivaltis H. 2009. Ayurveda and Panchakarma measuring the effects of a holistic health intervention. Scientific World Journal 9, 272–280.  https://doi.org/10.1100/tsw.2009.35

Crane E. 1990. Honey from honeybees and other insects. Ethology Ecology and Evolution 3(1), 100–105. https://doi.org/10.1080/03949370.1991.10721919

Davenport RK, Mossel DA, Skinner FD, Passinfre SM. 1980. Experience with some methods for the enumeration and identification of yeasts occurring in foods. In Biology and Activities of Yeasts, Academic Press London

Dhivya SM. Kalaichelvi K. 2017. UV-Visible Spectroscopic and FTIR Analysis of Sarcostemma brevistigma, Wight. and Arn. International Journal of herbal medicine  9(3), 46-49. https://doi.org/10.22159/ijcpr.2017.v9i3.18890

Doner LW, Honey Caballero  B, Trugo L. Finglas PM. (Eds). 2000. Encyclopedia of Food Sciences and Nutrition, Oxford: Academic Press, India

Edwards U, Rogall T, Blocker H, Emde M, Bottger EC. 1989. Isolation and direct complete nucleotide determination of entire genes: Characterization of a gene coding for16S ribosomal RNA. Nucleic Acids Research 17, 7843–7853.  https://doi.org/10.1093/nar/17.19.7843

Eekhof JA, Van Wijk B,  Knuistingh Neven A, van der Wouden JC. 2012. Interventions for ingrowing toenails. Cochrane Database of systematic reviews 4(4), 1-78. https://doi.org/ 10.1002/14651858.CD001541.pub3

Erlund. 2004. Review of the flavonoids quercetin, hesperetin, and naringenin. Dietary sources, bioactivities, bioavailability and epidemiology. Nutrition Research 24(10), 851–874. https://doi.org/10.1016/j.nutres.2004.07.005

Kamble V, Gaikwad N. 2016. Fourier transform infra-red spectroscopy spectroscopic studies in Embelia ribes Burm.F. A vulnerable medicinal plant. Asian Journal of Pharmaceutical and Clinical Research 9(3), 41-47.

Kanatt SR, Chander R, Sharma A. 2007. Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat. Food Chemistry 100, 451-458. https://doi.org/10.1016/j.foodchem.2005.09.066

Li H, Veenendaal E, Shukor NA, Cobbinah JR, Leifert C. 2008. Yeast populations on the tropical timber tree species Milicia excels. Letters in Applied Microbiology  21, 322-326. https://doi.org/10.1111/j.1472-765X.1995.tb01070.x

Molan PC. 1992. The antibacterial activity of honey. Bee world 73(1), 5-28. https://doi.org/10.1080/0005772X.1992.11099109

Nishimiki M, Appaji N, Yagi K. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochemical and Biophysical  Research Communications 46(2), 849- 854.  https://doi.org/10.1016/s0006-291x(72)80218-3

Nzeako BC, Hamdi J. 2000. Antimicrobial Potential of Honey on Some Microbial Isolates. Medical Sciences 2, 75-79.

O’Meara S, Al-Kurdi D, Ologun Y, Ovington LG, Martyn St James M, Richardson R. 2014. Antibiotics and antiseptics for venous leg ulcers. Cochrane Database of Systematic Reviews 1(1), 1-137.  https://doi.org/10.1002/14651858.CD003557.pub5

Oyaizu M. 1986. Studies on products of browning reaction: Antioxidative activity of product of browning reaction prepared from glucosamine. Japan Journal of Nutrition and dietetics 44, 307-315. http://dx.doi.org/10.5264/eiyogakuzashi.44.307

Radwan SA, El-Essawy M, Sarhan MM. 1984. Experimental evidence for the occurrence in honey of specific substances active against microorganisms. Zentral Mikrobiologie 39, 249-255.

Rao SR, Bhadra B, Kumar NN, Shivaji S. 2007. Candida hyderabadensis sp. nov., a novel ascomycetous yeast isolated from wine grapes. FEMS Yeast Research 7, 489-493. https://doi.org/10.1111/j.1567-1364.2006.00206.x

Sadasivam and Manickam A. 2004. Biochemical Methods, 2nd Edition, New Age International (P) Limited Publishers, New Delhi, India.

Sakihama YM, Cohen F, Grace SC, Yamasaki H. 2002. Plant phenolic antioxidant and prooxidant activities: Phenolics-induced oxidative damage mediated by metals in plants. Toxicology 177, 67-80. https://doi.org/10.1016/s0300-483x(02)00196-8

Spencer JFT, Spencer M. 1997. Yeasts in Natural and Artificial Habitats. Springer-Verlag, Heidelberg, Berlin.

Subramanian R, Hebbar H, Umesh, Rastogi NK. 2007. Processing of Honey: A Review. International Journal of Food Properties 10, 127–143.

Tournas VH. 2005. Moulds and yeasts in fresh and minimally processed vegetable and sprouts. International Journal of Food Microbiology 99(1), 71-77. https://doi.org/10.1016/j.ijfoodmicro.2004.08.009 

Xing R, Yu H, Liu S, Zhang W, Zhang Q, Li Z, Li P. 2005.  Antioxidant activity of differently regioselective chitosan sulfates In Vitro. Bioorganic and Medicinal Chemistry 13, 1387-1392. https://doi.org/10.1016/j.bmc.2004.11.002

Zar JH. 1984. In: Biostatistical Analysis, Englewood Cliffs, N.J.: Prenticehall, Inc, India.