Screening, isolation and evaluation of probiotic potential Lactobacillus acidophilus strains from available sources in Bangladesh

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Research Paper 07/07/2023
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Screening, isolation and evaluation of probiotic potential Lactobacillus acidophilus strains from available sources in Bangladesh

Ariful Haque, Saiful Haq, Dipa Roy, Zohorul Islam Moon
Int. J. Biosci.23( 1), 68-80, July 2023.
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Probiotics, live microorganisms that promote health by balancing the gut microbiota, have gained popularity in food and supplements. This study aimed to identify potential probiotic strains of Lactobacillus acidophilus isolated from available yoghurt/fermented food sources in Bangladesh. The research addressed the need for indigenous strains to cater to the local population’s health requirements in the face of imported probiotic products dominating the market. Eight yoghurt samples from Bogra District were collected and cultured using Man Rogosa and Sharp (MRS) broth and agar. The isolated lactobacilli were further characterised through sequencing, and the Lactobacillus acidophilus LA-5 strain was identified in all isolates. Lactobacillus acidophilus LA-5 is a probiotic strain that has been employed in food and dietary supplements. Additionally, a stock contamination test was conducted to ensure sample purity. In vitro tests were performed to assess the probiotic potential, including acid tolerance, bile salt tolerance, antibiotic sensitivity, and storage ability in order to mimic the gut environment and industrial processing. The LA-5 strain exhibited sensitivity to amikacin, gentamicin, and levofloxacin and the ability to grow after long-term storage at -80°C. These findings highlight the promising probiotic potential of indigenous strains in Bangladesh, specifically Lactobacillus acidophilus LA-5. The results can guide further research and contribute to the development of locally sourced probiotics tailored to the Bangladeshi population, which have been used by the local population for many years in fermented food.


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