Antimicrobial activity and antibiotic resistance of LAB isolated from Sudanese traditional fermented camel (Camelus dromedarius) milk gariss

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Research Paper 01/11/2012
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Antimicrobial activity and antibiotic resistance of LAB isolated from Sudanese traditional fermented camel (Camelus dromedarius) milk gariss

A. I. Ahmed, B. E. Mohamed, N.M. E. Yousif, B. Faye, G. Loiseau
Int. J. Biosci.2( 11), 129-136, November 2012.
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Abstract

Fourteen strains of Enterococcus species, 3 strains of Lactobacillus species and two strains of Streptococcus isolated from 6 different gariss samples collected from Kordofan and Khartoum production sites, Sudan were screened for antibacterial activity against Staphylococcus aureus ATCC 25923 and E.coli ATCC25922 and for their antibiotic resistance against 9 antibiotics. The results indicated that all the 19 strains of LAB isolated from gariss were found to show no antimicrobial effect on both Staphylococcus aureus ATCC 25923 and E.coli ATCC25922. As intrinsic resistance of Enterococci for antibiotics, there were 57.14% (8 isolates) showing resistance to at least one antibiotic and some even to almost all antibiotics studied, one strain (E. durans R05) resistant to penicillin. S. acidominimus was resistant to only Ciproflaxin while S. thermophilus was resistant to Chloramphenicol, Erythromycin and Ciproflaxin; L. plantarum BJG32 was resistant Chloramphenicol, Erythromycin and Ciproflaxin; L. pentosus S2LPO2 was resistant Erythromycin and Ciproflaxin, while L. plantarum BJ6 was not resistant to any one of antibiotics investigated..

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Adams MR, Marteau P .1995. On the safety of lactic acid bacteria from food. International Journal of Food Microbiology 27, 263– 264.

Ahn C, Thompson DC, Duncan C, Stiles ME.1992. Mobilization and location of the genetic determinant of chloramphenicol resistance from Lactobacillus plantarum ca TC2R. Plasmid 27, 169– 176.

Akpinar A, Yerlikaya O, Kilic S. 2011. Antimicrobial activity and antibiotic resistance of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus strains isolated from Turkish homemade yoghurts. African Journal of Microbiology Research Vol. 5(6), 675-682.

Ammor S, Tauveron G, Dufour E, Chevallier I. 2006. Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility, 1—Screening and characterization of the antibacterial compounds. Food Control 17, 454–461.

Benkerroum N, Mekkaoui M, Bennani N, Hidane K. 2004. Antimicrobial activity of camel’s milk against pathogenic Strains of E. coli and Listeria monocytogenes. International Journal of Dairy Technology Vol, 57: No 1.

Charteris WP, Kelly PM, Morelli L, Collins JK. 1998. Antibiotic susceptibility of potentially probiotic Lactobacillus species. Journal of Food Protection 61, 1636–643.

Clementi F, Aquilanti L. 2011. Recent investigations and updated criteria for the assessment of antibiotic resistance in food lactic acid bacteria. Anaerobe, doi:10.1016/j.anaerobe.2011.03.021.

Curragh HJ, Collins MA. 1992. High levels of spontaneous drug resistance in Lactobacillus. Journal of Applied Bacteriology 73, 31– 36.

Ebrahimi MT, Ouwehand AC, Hejazi MA , Jafari P. 2011. Traditional Iranian dairy products: A source of potential probiotic lactobacilli. African Journal of Microbiology Research 5(1), 20-27.

Fons M, Hege T, Ladire M, Raibaud P, Ducluzeau R, Maguin E. 1997. Isolation and characterization of a plasmid from Lactobacillus fermentum conferring erythromycin resistance. Plasmid 37, 199– 203.

Herreros MA, Sandoval H, Gonzalez L, Castro JM, Fresno JM, Tornadijo ME. 2005. Antimicrobial activity and antibiotic resistance of lactic acid bacteria isolated from Armada cheese (a Spanish goats’ milk cheese). Food Microbiology 22, 455–459.

Hidron AI, Edwards JR, Patel J, Horan TC, Sievert DM, Pollock DA. 2009. NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006–2007. Infect Control Hosp Epidemiol 2008. 29; 996–1011.

Ishiwa H, Iwata S. 1980. Drug resistance plasmids in Lactobacillus fermentum. Journal of General and Applied Microbiology 26, 71– 74.

Jay J M. 1982. Antimicrobial properties of diacetyl. Applied and Environmental Microbiology 44, 525– 532.

Kalalou I, Faid M, Ahami AT. 2004. Extending shelf life of fresh minced camel meat at ambient temperature by Lactobacillus delbrueckii subsp. Delbrueckii. Electric Journal of Biotechnology 7, 243–248.

Kelly WJ, Asmundson R V, Huang CM. 1996.Isolation and Charcterisation of bacteriocin-producing lactic acid bacteria from ready-to-eat food products. International Journal of food Microbiology 33, 209-218.

Khali RK. 2009. Evidence for probiotic potential of a capsular producing Streptococcus thermophilus CHCC 3534 Strain. African Journal of Microbiology Research 3 (1), 27-34.

Klaenhammerv T R. 1988. Bacteriocins of lactic acid bacteria. Biochimie 70, 337–349.

Lindgren SE, Dobrogosz WJ. 1990. Antagonistic activities of lactic acid bacteria in food and feed fermentations. FEMS Microbiology Reviews 7, 149–163.

Liu G, Griffiths MW, Wu P, Wang H Zhang J, Li P. 2011. Enterococcus faecium LM-2, a multi-bacteriocinogenic strain naturally occurring in “Byaslag”, a traditional cheese of Inner Mongolia in China. Food Control 22, 283-289.

Majhenič AC, Matijašic BB. 2001. Antibiotics influence on lactic acid bacteria inhabiting gastrointestinal tract. Mljekarstvo 51 (2), 119-134.

Mathur S, Singh R. 2005.Antibiotic resistance in food lactic acid bacteria, A review. International Journal of Food Microbiology 105, 281– 295.

Ogier JC, Serror P. 2008. Safety assessment of dairy microorganisms: the Enterococcus genus. Int J Food Microbiol 126, 291-301.

Ogunbanwo ST. 2005. Functional properties of lactic acid bacterial isolated from ogi and fufu, Two Nigerian fermented foods. J food Science 27, 14-21.

Piard JC, Desmazeaud M .1991. Inhibiting factors produced by lactic acid bacteria: 1. Oxygen metabolites and catabolism end products. Lait 71, 525–541.

Piard JC, Desmazeaud M. 1992. Inhibiting factors produced by Lactic acid bacteria: 2. Bacteriocins and other antibacterial substances. Lait 72, 113–142.

Rathnayaka RMUSK. 2012. Effect of Bacterial interactions on Antimicrobial compound production by Lactic acid bacteria. International Journal of Dairy Science 7(3), 63-69.

Salminen S, Von Wright A, Morelli L, Marteau P, Brassart D, Vos de WM, Fonde’n R, Saxelin M, Collins K, Mogensen G, Birkeland SE, Sandholm TM. 1998. Demonstration of safety of probiotics. International Journal of Food Microbiology 44, 93– 106.

Standardisation de l’Antibiogramme en Médecine Vétérinaire. 2008. Standardisation de l’antibiogramme en médecine vétérinaire à l’échelle nationale, selon les recommandations de l’OMS, 4 éme édition, République Algérienne Démocratique et Populaire, Ministère de l’Agriculture et du Développement Rural, Ministère de la santé de la population et de la Reforme Hospitalière, Selon les recommandations de l’OMS.

Tannock GW, Luchansky JB, Miller L, Connell H, Thode- Andersen S, Mercer AA, Klaenhammer TR. 1994. Molecular characterization of a plasmid-borne (pGT633) erythromycin resistance determinant (ermGT) from Lactobacillus reuteri 100- 63. Plasmid 31, 60– 71.

Yousif NME. 2003. Molecular typing of lactic acid bacteria associated with Hussuwa A Sudanese fermented Food, PhD thesis, University of Khartoum, Khartoum north, Shambat, Sudan.

Zhou JS, Pillidge CJ, Gopal PK, Gill HS. 2005. Antibiotic susceptibility profiles of new probiotic Lactobacillus and Bifidobacterium strains. Int. J. of Food Microbiology 98, 211 – 217.

Zhou N, Zhang JX, Fan MT, Wang J Guo G, Wei XY. 2012. Antibiotic resistance of lactic acid bacteria isolated from Chinese yogurts. J. Dairy Science 95, 4775–4783.