Antioxidant, antibacterial activities and phenolic content of organic fractions obtained from Chamaerops humilis L. leaf and fruit
Paper Details
Antioxidant, antibacterial activities and phenolic content of organic fractions obtained from Chamaerops humilis L. leaf and fruit
Abstract
The use of plant extracts are the subject of numerous studies because, in addition to their use as preservatives in foodstuffs by replacing synthetic antioxidants, they are involved in the treatment of many diseases. This study investigated the antioxidant and antibacterial activities of Chamaerops humilis L. (Asteraceae) leaf and fruit, collected in north east of Algeria and their respective fractions, obtained using dichloromethane (DCMF), ethyl acetate (EAF), n-butanol (BF) and water (WF). The total phenolic and total flavonoids content was determined by the Folin-Ciocalteu and the aluminum chloride (2%) reaction, respectively. Antioxidant activity was measured, using different antioxidant assays (DPPH, FRAP, BCB). The antibacterial activities of the fractions were tested on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterococcus faecalis and Salmonella typhimurium. Two different methods were employed for the determination of antibacterial activities: disc diffusion method and minimum inhibitory concentrations (MICs). A significant correlation existed between concentrations of the extract and antioxidant activity since the maximum antioxidative activities were exhibited by ethyl acetate fraction [IC50=0.12 mg/ml (DPPH assay)] which has the highest levels of phenolic compounds (214,8±3,26 mg GAE/g fractions).Concerning antibacterial activity , the lowest activity was founded on the (WF)from fruits against Salmonella typhimurium (MIC=70mg/ml)and the highest was found on the (EA) fraction from leaves against Enterococcus faecalis (MIC=0,25 mg/ml) . These findings demonstrate the remarkable potential of this plant as new source of antioxidant ingredient with interesting antibacterial capacities.
Aksoy L, Kolay E, Agılonu Y, Aslan Z, Kargıoglu M. 2013. Free radical scavenging activity, total phenolic content, total antioxidant status, and total oxidant status of endemic Thermopsis turcica. Saudi Journal of Biological Sciences 20, 235–239. http://dx.doi.org/10.1016/j.sjbs.2013.02.003
Al Akeel R, Al-Sheikh Y, Mateen A, Syed R, Janardhan K, Gupta VC. 2014. Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains. Saudi Journal of Biological Sciences 21, 147–151. http://dx.doi.org/10.1016/j.sjbs.2015.08.006
Al-Fatimi M, Wurster M, Schröder G, Lindequist U. 2007. Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from Yemen. Journal of Ethnopharmacology 111, 657-666. http://dx.doi.org/10.1016/j.jep.2007.01.018
Arvouet-Grand A, Vennat B, Pourrat A, Legret P. 1994. Standardisation d’un extrait de propolis et identification des principaux constituants. Journal de pharmacie de Belgique 49, 462–468.
Beghalia M, Ghalem S, Allali H, Belouatek A, Marouf A. 2008. Inhibition of calcium oxalate monohydrate crystal growth using Algerian medicinal plants. Journal of Medicinal Plants Research 2, 66-70.
Benhammou N, Bekkara FA, Panovska TK. 2009.Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus. Comptes Rendus Chimie 12, 1259-66. http://dx.doi.org/10.1016/j.crci.2009.02.004
Benmehdi H, Hasnaoui O, Benali O, Salhi F.2012. Phytochemical investigation of leaves and fruits extracts of Chamaerops humilis L. Journal of Materials and Environmental Science 3(2), 320-237.
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199–1200.
Blumenthal M, Goldberg A, Brinckmann J. 2000. Herbal Medicine: Expanded Commission E Monographs. Austin, Texas, American Botanical Council; Boston, Integrative Medicine Communications, p.640.
Bouacha M, Berredjem H, Berredjem M, Bouzerna N. 2015. In-vitro Antibacterial Activity of two Novel Sulfonamide Derivatives against Urinary Strains of Escherichia coli. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 6(1), Page No. 770.
Bouterfas K, Mehdadi Z, Elaoufi MM, Latreche A, Benchiha W. 2016.Antioxidant activity and total phenolic and flavonoids content variations of leaves extracts of white Horehound (Marrubium vulgare Linné) from three geographical origins. Annales Pharmaceutiques Françaises. http://dx.doi.org/10.1016/j.pharma.2016.07.002
Chan KW, Iqbal S, Khong NMH, Ooi DJ, Ismail M. 2014. Antioxidant activity of phenolics and saponins rich fraction prepared from defatted kenaf seed meal. LWT – Food Science and Technology 56, 181-186. http://dx.doi.org/10.1016/j.lwt.2013.10.028
Chew YL, Lim YY, Omar M, Khoo KS. 2008. Antioxidant activity of three edible seaweeds from two areas in South East Asia. LWT- Food Science and Technology41, 1067–1072. http://dx.doi.org/10.1016/j.lwt.2007.06.013
CLSI. 2010. Performance standards for antimicrobial susceptibility testing: 20th informational supplement. CLSI document M100- S20.Clinical and Laboratory Standards Institute, Wayne.
Dhingra N, Kar A, Sharma R, Bhasin S. 2017. In-vitro antioxidative potential of different fractions from Prunus dulcis seeds: Vis a visa ntiproliferative and antibacterial activities of active compounds. South African Journal of Botany. 108, 184–192. http://dx.doi.org/10.1016/j.sajb.2016.10.013
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. 2006. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry.97, 654–660. http://dx.doi.org/10.1016/j.foodchem.2005.04.028
Falleh H, Ksouri R, Chaieb K, Bouraoui NK, Trabelsi N, Boulaaba M, AbdellyC. 2008. Phenolic composition of Cynara cardunculus L. organs, and their biological activities. Comptes Rendus Biologies. 331, 372–379. http://dx.doi.org/10.1016/j.crvi.2008.02.008
Guzmán B, Fedriani JM, l Delibes M, Vargas P. 2017. The colonization history of the Mediterranean dwarf palm (Chamaerops humilis L., Palmae). Tree Genetics & Genomes.13:24. http://dx.doi.org/10.1007/s11295-017-1108-1
Hammer KA, Carson CF, Riley TV. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology 86, 985-990.
Khadraoui A, Hachama K, Khodja M, Khelifa A , Mehdaoui R, Harti H, Abid S , Agnieszka Najda B, Chahboun N. 2015 .Extraction study and the antibacterial activity of phenol and flavonoid contents in Mentha pulegium L. from Algeria. Journal of Materials and Environmental Science. 6(9), 2501-2508.
Kulisic T, Radonic A, Katalinic V, Milos M. 2004. Use of different methods for testing antioxidant activity of oregano essential oil. Food Chemistry. 85, 633-640.
Li HB, Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y. 2007.Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chemistry 102, 771–776.
LI WJ, Cheng XL, Liu J, Wang GL, Lin RC, Du SS, Liu ZL. 2012. Phenolic Compounds and Antioxidant Activities of Liriope muscari. Molecules. 17, 1797-1808. http://dx.doi.org/10.3390/molecules17021797
Lopes-Lutz D, Alviano DS, Alviano CS, Kolodziejczyk PP. 2008.Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry.69, 1732–1738.
Martins S, Amorim ELC, PeixotoSobrinho TJS. 2013. Antibacterial activity of crude methanolic extract and fractions obtained from Larrea tridentata leaves. Industrial Crops and Products. 41, 306– 311.
Matkowski A, Tasarz P, Szypuła E. 2008. Antioxidant activity of herb extracts from five medicinal plants from Lamiaceae, subfamily Lamioideae. Journal of Medicinal Plants Research. 11, 321-330.
Michel T, Destandau E, Le Floch G, Lucchesi ME, Elfakir C. 2011. Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (Hippophaë rhamnoides L.) leaf, stem, root and seed. Food Chemistry. 131, 754–760.
Muanda FN, Soulimani R, Diop B, Dicko A. 2011.Study on chemical composition and biological activities of essential oil and extracts from Stevia rebaudiana Bertoni leaves. LWT- Food Science and Technology 44, 1865-1872. http://dx.doi.org/10.1016/j.lwt.2010.12.002
NCCLS. (National Committee for Clinical Laboratory Standards). 2000. Performance standards for antimicrobial disc susceptibility tests. Approved Standard, M2-A7.
Olivier MT, Muganza FM, Shai LJ, Gololo SS, Nemutavhanani LD. 2017. Phytochemical screening, antioxidant and antibacterial activities of ethanol extracts of Asparagus suaveolens aerial parts. South African Journal of Botany 108, 41–46. http://dx.doi.org/10.1016/j.sajb.2016.09.014
Ordonez AAL, Gomez JD, Vattuone MA, Isla MI. 2006. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chemistry 97(3), 452-458. http://dx.doi.org/10.1016/j.foodchem.2005.05.024
Oyaizu M. 1986. Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition 44, 307–315.
Pratt DE. 1980. Natural antioxidants of soybean and other oil-seeds. In M. G. Simic, & M. Karel (Eds.), Autoxidation in food and biological systems (283–292 p). New York: Plenum Press.
Ravikumar YS, Harish BG, Krishna V, Vaidya VP, Mahadevan KM. 2007. Antibacterial activity of stem bark constituents of Polyalthia cerasoides (Roxb.)Bedd. International journal of biomedical and pharmaceutical sciences 1(2), 164-167.
Sacchetti G, Maietti S, Mussoli M, Scaglianti M, Manfredini S, Radice M, Bruni R. 2005. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chemistry 91, 621–632. http://dx.doi.org/10.1016/j.foodchem.2004.06.031
Salem JH, Chevalot I, Harscoat‑Schiavo C, Paris C, Fick M, Humeau C. 2010.Biological activities of flavonoids from Nitraria retusa (Forssk.) Asch and their acylated derivatives. Food Chemistry 124, 486‑494, 2011.
Singleton VL, Rossi JA. 1965. Colorimetryof total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16, 144–153.
Tiwari U, Jadon M, Nigam D. 2014.Evaluation of antioxidant and antibacterial activities of methanolic leaf extract of Callistemon viminalis. International Journal of Pharmaceutical Sciences and Business Management 2, 01-12.
Saber Belhaoues, Sandra Amri, Mourad Bensouilah, Ratiba Seridi (2017), Antioxidant, antibacterial activities and phenolic content of organic fractions obtained from Chamaerops humilis L. leaf and fruit; IJB, V11, N1, July, P284-297
https://innspub.net/antioxidant-antibacterial-activities-and-phenolic-content-of-organic-fractions-obtained-from-chamaerops-humilis-l-leaf-and-fruit/
Copyright © 2017
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0