Assessment of phytochemical constituents, antioxidant activity, cytotoxicity and antiproliferative activity of apricot
Paper Details
Assessment of phytochemical constituents, antioxidant activity, cytotoxicity and antiproliferative activity of apricot
Abstract
Medicinal herbs are having decisive role to improve the human health standard because of predominant bioactive constituents. Prunus armeniaca (Apricot) is a highly nutritious fruit with a rich composition of health promoting components with a unique taste. Apricot samples grown in Gilgit Baltistan were assessed for their biochemical attributes, FTIR interferogram profiling, Phytochemicals screening, antioxidant activity, Cytotoxicity, and MTT based antiproliferative activity. HPLC data of fruit extract showed the presence of phenolic acids and flavonoid contents, appreciable quantities of vanillic acid, gallic acid and catechin. These secondary metabolites exhibited rich biochemical and functional activity, significant antioxidant potential and free radicals scavenging activity. FTIR spectra’s were used to obtain information on functional groups and chemical structure of apricot samples showed significant antioxidant activity as well antiproliferative activity against cervical cancer cells (HeLa-S3). Anti-cancer results are also supported by antitumor potato disc assay by significant inhibition of tumor formation.
Akindahunsi AA, Oyetayo FL. 2006. Nutrient and antinutrient distribution of edible mushroom Pleurotus tuberregium (Fries) Singer. Elsevier 39, 548-553.
Ali S, Masud T, Abbasi KS. 2011. Physico-chemical characteristics of apricot (Prunus armeniaca L.) grown in Northern Areas of Pakistan. Scientia Horticulture 130, 386-392.
Arnao MB, Cano A, Acosta M. 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry 7, 239-244.
Association of Official Analytical Chemists – AOAC. 2005. Official Methods of Analysis of the Association of Official Analytical Chemists. (Ed.,18th)., Gaithersburg AOAC.
Chen CY, Blumberg JB. 2008. Phytochemical composition of nuts. Asia Pacific Journal of Clinical Nutrition 17(1), 329-332.
Dag A, Kerem Z, Yogev N, Zipori, Lavee S, and Ben-David E. 2011. Influence of time of harvest and maturity index on olive oil yield and quality. Scientia Horticulturae 127(3), 358-366.
Dragović-Uzelac V, Savić Z, Brala A, Levaj B, Kovačević and Biško A. 2010. Evaluation of phenolic content and antioxidant capacity of blueberry cultivars (Vaccinium corymbosum L.) grown in the Northwest Croatia. Food Biotechnology 48(2), 214-221.
Ferrigini NR, Putna JE, Anderson B, Jacobsen LB, Nichols DE, Moore DS, McLaughlin JL. 1982. Modification and evaluation of the potato disc assay and antitumor screening of Euphorbia seeds. Journal of Natural Products 45, 679-686.
Galsky AG, Wilsey JP, Powell RG. 1980. Crown gall tumor disc bioassay. Plant Physiology 65, 184-185.
Hussain J, Khan AL, Rehman NU, Hamayun M, Shah T, Nisar M, Bano T, Shinwari ZK, Lee IJ. 2009. Proximate and nutrient analysis of selected vegetable species: A case study of Karak region, Pakistan. African Journal of Biotechnology 8(12), 2725-2729.
Kanegusuku M, Benassi JC, Pedrosa RC, Yunes RA, Filho VC, Maia AA, Marcia M, Monache FD, Niero R. 2001. Cytotoxic, hypoglycemic activity and phytochemical analysis of Rubus imperialis (Rosaceae). Naturforsch 57, 272-276.
Kaneria M, Kanari B, Chanda S. 2012. Assessment of effect of hydroalcoholic and decoction methods on extraction of antioxidants from selected Indian medicinal plants. Asian Pacific Journal of Tropical Biomedicine 2(3), 195-202.
McCune LM, Johns T. 2002. Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the North American boreal forest. Ethnopharmacology 82, 197-205.
McLaughlin JL. 1991. Crown-gall Tumors in Potato Discs and Brine Shrimp Lethality: Two Simple Bioassays for Higher Plant Screening and Fractionation. Methods in Plant Biochemistry 39, 387-396.
Meyer-Alber A, Hartmann H, Sumpel F, Creutzfeldt W. 1992. Mechanism of insulin resistance in CCl4-induced cirrhosis of rats. Gastroenterology 102, 223-229.
Molyneux P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Journal of Science and Technology 26(2), 211-219.
Muthukumaran P, Padmapriya P, Salomi S, Umamaheshwari R, Kalaiarasan P, Malarvizhi C. 2011. In Vitro Anti-Microbial Activity of Leaf Powder, Asian Journal of Pharmacology 1(4), 108-110.
Sass-Kiss A, Kiss J, Milotay P, Kerek MM, Toth-Markus M. 2005. Differences in anthocyanin and carotenoid content of fruits And vegetables. Food Research International 38, 1023-1029.
Sultana B, Anwar F, Przybylski. 2007. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica and Eugenia jambolana. Food Chemistry 104, 1106-1114.
Tokusoglu O, Unal MK, Yemis F. 2005. Determination of phytoalexin in peanut and pistachios by HPLC-DAD and GC-MS. Journal of Agriculture Food Chemistry 53, 5003-5009.
Vinson JA, Zubik L, Bose P, Samman N, Proch J. 2000. Dried fruits. Excellent in vitro and in vivo antioxidants. Journal of the American College of Nutrition 24, 44-50.
Yigit H, Queenan AM, Anderson GJ, Domenech-Sanchez A, Biddle JW, Steward CD Tenover FC. 2001. Novel carbapenem-hydrolyzing b-lactamase, KPC-1, from a Carbapenem resistant strain of Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy 45, 1151-1161.
Zhao J, Zhao Y. 2004. Effects of curcumin on proliferation and apoptosis of human cervical carcinoma HeLa cells in vitro. Chinese Journal of Cancer Research 16, 225-8.
M. Asghar, M. Gulfraz, M. J. Asad, T. Masud (2018), Assessment of phytochemical constituents, antioxidant activity, cytotoxicity and antiproliferative activity of apricot; IJB, V12, N6, June, P346-354
https://innspub.net/assessment-of-phytochemical-constituents-antioxidant-activity-cytotoxicity-and-antiproliferative-activity-of-apricot/
Copyright © 2018
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0