Assessment of some polymers for the formulation of rhizobial liquid inoculants
Paper Details
Assessment of some polymers for the formulation of rhizobial liquid inoculants
Abstract
The present study was conducted to screen and evaluate four polymeric additives, polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), polyethylene glycol (PEG) and Gum Arabic at different concentrations for their ability to maintain and support bacterial viability of rhizobial strains TAL 380, USDA 110 and local isolates ENRRI 12 and ENRRI 1 during the initial period of growth compared with growth in YEM medium without additives. The comparison of the different concentrations for each polymer was carried out after 8 days of incubation and Data were expressed as log number of Colony Forming Units (CFU) ml-1. The results of the study clearly indicate that most of the tested liquid formulations supported an adequate survival of rhizobial strains providing more than 108 bacteria ml-1 at the end of the experiment. Liquid inoculants formulated with Gum Arabic at 0.1%, 0.5% and 0.8% promoted long term survival of ENRRI 1, ENRRI 12 and TAL 380 while PVP at 0.1% supported the growth of strain USDA 110. The selected appropriate concentrations of the polymers for each rhizobial strain and isolate were then recommended for further studies concerning their uses as liquid inoculants. Few concentrations tended to slightly reduce cell density when compared to growth in YEM media. The study confirms the better survival of rhizobial cells in liquid inoculants amended with different concentrations of polymeric additives compared to liquid broth medium during initial growth. In addition, a degree of interaction between strains of rhizobia and polymeric additives was also noticed.
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