In-vitro evaluation of aggressiveness and chlorothalonil sensitivity in Taphrina deformans isolates from peach leaf curl disease in Swat
Paper Details
In-vitro evaluation of aggressiveness and chlorothalonil sensitivity in Taphrina deformans isolates from peach leaf curl disease in Swat
Abstract
Peach (Prunus persica L.), a significant fruit crop in Swat, is frequently afflicted by peach leaf curl, a disease caused by the fungus Taphrina deformans. This study evaluates the in-vitro responses of six isolates of T. deformans to normal and chemical stress conditions. Isolates were cultured on Yeast Extract Peptone Dextrose Agar (YEPDA), both unamended and amended with 500 mg/L Chlorothalonil, with three replicates per condition. The colony diameter of each isolate was measured bi-weekly for two weeks. Disease incidence in peach orchards varied significantly across districts, with the highest recorded in Asharay (91.53%) and the lowest in Matta (21.34%). In unamended medium, Isolate Td5 (Matta) exhibited the highest aggressiveness, followed by Td3 (Null), while Td2 (Baidara) was the least aggressive. In the amended medium, Isolate Td6 (Bodigram) was the most aggressive, with Td5 (Matta) also showing high aggressiveness, and Td3 (Null) was the least aggressive. Sensitivity to Chlorothalonil varied among isolates; Td4 (Sambat) and Td3 (Null) were more sensitive, whereas Td6 (Bodigram) was the least sensitive. These findings highlight the variability in aggressiveness and fungicide sensitivity among T. deformans isolates, underscoring the need for tailored management strategies, including the strategic application of Chlorothalonil, to effectively control peach leaf curl in affected regions.
Agricultural Statistics of Pakistan. 2010–2011. Peach production and area. Table 51, p. 95. Retrieved from: http://www.pbs.gov.pk/content/agricultural-statistics-pakistan-2010-11.
Anonymous. 1998. Food and Agriculture Organization. FAO production Yearbook.
Bassi D, Monet R. 2008. Botany and taxonomy. In: Layne DR, Bassi D (eds). The Peach: Botany, Production, and Uses. CABI, Cambridge USA, p. 1-36.
Celiński R, Krzemińska B, Grzywa-Celińska A, Szewczyk G, Szewczyk KD. 2024. A review on the potential use of medicinal plants from the Apiaceae and the Rosaceae families in cardiovascular diseases—experimental evidence and traditional applications. Applied Sciences 14(9), 3728.
Chuadhary MI, Malik MN. 1994. Fruit crops in horticulture. First Ed. National Book Foundation, Islamabad, p. 471-473.
Ferguson B, Hldelorand R, Hespendheild G. 1987. All about growing fruits, berries and nuts. Ortho Books, San Francisco, CA, p. 287.
Ingham R, McMorran J, Ocamb CM, Pscheidt JW, Putnam M. 2010. Peach leaf curl. In: Pacific Northwest Plant Disease Management Handbook: An Online Guide to Plant Disease Control. Corvallis: Oregon State University. Accessed January.
Jahan Z, Khan Z. 2008. Peach marketing in NWFP. Sarhad Journal of Agriculture 24(2), 161-168.
Jan MT, Shah P, Hollington PA, Khan MJ, Shohail Q. 2009. Agriculture Research: Design and Analysis. 1st ed. Dept. of Agronomy, KPK Agricultural University Peshawar, Pakistan.
Jim K, Stein L, Nesbitt M. 2001. Extension fruit specialists. Texas Agricultural Life Extension.
Karim MM, Usman HM, Tan Q, Hu JJ, Fan F, Hussain R, Luo CX. 2024. Fungicide resistance in Colletotrichum fructicola and Colletotrichum siamense causing peach anthracnose in China. Pesticide Biochemistry and Physiology 203, 106006.
Khattak MS, Malik MN, Khan MA. 2002. Guava propagation via in vitro technique. Sarhad Journal of Agriculture 18(2), 199-202.
McCain AH, Perry EJ, Hickman GW. 1979. Leaf curl fungicides. California Journal of Plant Pathology 46, 1-2.
McManus P, Hudelson B. 2004. Peach leaf curl. University of Wisconsin Garden Fact Sheets. Retrieved from https://pddc.wisc.edu/2015/07/30/peach-leaf-curl.
Moller WJ, McCain AH, Chaney DH. 1979. Leaf curl control in peaches and nectarines. California Agriculture National Research Leaflet, 2613. Oakland: University of California.
Mustapha SB, Abdallah D, Haffar S, Hannachi AS, Baraket G. 2024. Prunus species diversity, structure, and phylogenetic studies assessed by chloroplastic microsatellite markers. Iranian Journal of Science 27, 1-2.
Ogawa JM, Zehr EI, Bird GW, Ritchie DF, Uriu KI, Uyemoto JK. 1995. Compendium of stone fruit diseases.
USDA National Nutrient Data Base. 2016. Retrieved from: http://www.nutrition-and-you.com/peaches.html
Yancovic-Pakarati S, Moreno-Pakarati C, Seelenfreund D, Seelenfreund A, Castro MH, Hinrichsen P. 2024. Patrimonial grapevine varieties (Vitis vinifera L.) from Rapa Nui: genetic characterization and relationship with continental cultivars. New Zealand Journal of Botany 23, 1-8.
Mati Ullah, Shafee Ur Rehman, Kudaibergen Osmonaliev, 2024. In-vitro evaluation of aggressiveness and chlorothalonil sensitivity in Taphrina deformans isolates from peach leaf curl disease in Swat. Int. J. Biosci., 25(3), 58-63.
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