Isolation and Characterization of Probiotic Lactic Acid Bacteria from Human Saliva
Paper Details
Isolation and Characterization of Probiotic Lactic Acid Bacteria from Human Saliva
Abstract
Probiotics, which are non-pathogenic microorganisms, interact with the gastrointestinal microbiota and offer various health benefits. These include boosting the host’s immune response, acting as antiallergic agents, and exhibiting antimicrobial, anti-cancer, and anti-inflammatory properties. Probiotics are also capable of restoring the disrupted microbiome in a dysbiotic gut. While they can be isolated from different environments, it is often recommended that probiotics intended for human use should be sourced from human origins. The present study shows the successful isolation and identification of lactic acid bacteria from saliva. The lactic acid bacteria were isolated from the collected saliva samples using MRS medium. The isolated bacterial strains were tested for hemolytic activity to verify their non-pathogenic nature. Further, the strains were partially identified by biochemical and microscopic observations; afterwards, the bacterial isolates, which showed non-hemolytic, were tested for their resistance potential against the standard antibiotics. The observed result shows that among the 94 individual isolates, only 12 showed non-hemolytic activity on the blood agar medium. Moreover, the isolated lactic acid bacteria belong to the Lactobacilli genus. The tested lactic acid bacterial strains almost showed resistant patterns against many tested antibiotics. The study’s findings demonstrate the variety of microbial species in human saliva. Given that these strains are derived from humans, they are likely to exhibit peak efficiency in applications related to food and pharmaceuticals designed for human consumption.
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Veenayohini Kumaresan, Ganesh Punamalai (2024), Isolation and Characterization of Probiotic Lactic Acid Bacteria from Human Saliva; IJB, V25, N1, July, P13-22
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