Toxicity activity of Azadirachta indica and Murraya koenigii against Aedes albopictus larvae in laboratory conditions

Paper Details

Research Paper 01/09/2015
Views (283) Download (6)
current_issue_feature_image
publication_file

Toxicity activity of Azadirachta indica and Murraya koenigii against Aedes albopictus larvae in laboratory conditions

Muthulinggam Nishan, Partiban Subramanian
J. Bio. Env. Sci.7( 3), 27-35, September 2015.
Certificate: JBES 2015 [Generate Certificate]

Abstract

Dengue serves as major threat to human beings and has caused an increase in the rate of mortality every year without considering age, gender or ethnicity. Thus, an alternative by using botanical extracts against the larvae of this mosquito was carried out. Azadirachta indica and Murraya koenigii were used to evaluate the larvacidal activity against 3rd instar of Aedes albopictus larvae. The bioassay was carried out by using the methanolic extract of the A. indica and M. koenigii and the combined activity was done with the ratio 1:1 of the plant extracts. The result showed that at 3.75 mg/ml A.indica exhibits highest larvacidal activity by providing 96.30% of mortality. Furthermore, M. koenigii showed the percentage of mortality of 83.70% at 3.75mg/ml. In the basis of median lethal concentration (LC50), it was found that the LC50 for A. indica was 1.45 mg/ml which is more susceptible than M. koenigii with 2.01 mg/ml as a LC50 value. The combination test showed that interaction between the 2 plants extract as an additivity effect. In nut shell, A. indica and M. koenigii was found to exhibit strong larvacidal activity. Thus, these plants have high potential to be developed as a biopesticide to control the emergence of A. albopictus larvae.

VIEWS 14

Abbott WS. 1925. A method of computing the effectiveness of insecticide. Journal of economic entomology 18, 265-267.

Alouani A, Rehimi N, Soltani N. 2009. Larvicidal activity of a neem tree extract (Azadirachtin) against mosquito larvae in the Republic of Algeria. Jordan Journal of Biological Sciences 2, 15-22.

Aarati N, Ranganath NN, Soumya GB, Kishore B, Mithun K. 2011. Evaluation of antibacterial and anticandidal efficacy of aqueous and alcoholic extract of Neem (AzadirachtaIndica) an in vitro study. International Journal of Research in Ayurveda and Pharmacy 2, 230-235.

Ajay S, Rahul S, Sumit G, Paras M, Mishra A, Gaurav A. 2011. Comprehensive review: Murraya koenigii Linn. Asian Journal of Pharmacy and Life Sciences 1, 417-425.

Arivoli S, Tennyson S. 2012. Larvicidal efficacy of Strychnosnuxvomica Linn. (Loganiaceae) leaf extracts against the filarial vector Culex quinquefasciatus Say (Diptera :Culicidae). World Journal of Zoology 7, 06-11.

Bansal P, Bansal R, Gupta V. 2010. Antifertility effects of Azadirachta indica (Neem) – A review. Annals of Biological Research 1, 108-113.

Bilal H, Akram W, Hassan SA. 2012. Larvicidal activity of Citrus Limonoids against Aedes albopictus larvae. Journal of Arthropod- Borne Diseases 6, 104-111.

Bhandri PR. 2012. Curry leaf (Murraya koenigii) or cure leaf: Review of its curative properties. Journal of Medical Nutrition and Nutraceuticals 1, 92-97.

Caminade C, Medlock JM, Ducheyne E, McIntre KM, Leach S, Baylis M, Morse AP. 2012. Suitability of European climate for the Asian tiger mosquito Aedes albopictus: recent trends and future scenarios. Journal of The Royal Society Interface 9, 1-10. http://dx.doi.org/10.1098/rsif.2012.0138

Chandrawathani P, Shariman ZY, Premaalatha B, Rahimah H, Norhafiza NH, NurulAini R, NorAndilla I, Wahab AR. 2013. Evaluation of neem leaf (Azadirachta indica) product for worm control on goats. Malaysian Journal of Veterinary Research 4, 5-12.

Cheah WK, Ng KS, Marzilawati AR, Lum LCS. 2014. A review of dengue research in Malaysia. Medical Journal of Malaysia 69, 59-67.

Darvekar VM, Patil VR, Choudhari AB. 2011. Anti-inflammatory activity of Murraya koenigii Spreng on experimental animals. Journal of Natural Product and Plant Resources 1, 65-69.

Gomase PV, Rangari VD, Verma PR. 2011. Phytochemical evaluation and hepatoprotective activity of fresh juice of young stem (tender) bark of Azadirachta indica A. Juss.International Journal of Pharmacy and Pharmaceutical Sciences 3, 55-59.

Hima CR, Manimegalai M. 2014. Studies on the control of mosquito, Aedes aegypti (Culicidae: Diptera) using the chloroform leaf extract of Murraya koenigii as biocide. World Journal of Pharmacy and Pharmaceutical Sciences 3, 766-773.

Kaplan L , Kendell D, Robertson D, Livdahl T, Khatchikian C. 2010. Aedes aegypti and Aedes albopictus in Bermuda: extinction, invasion and extinction. Biological invasions 12, 3277-3288. http://dx.doi.org/10.1007/s10530-010-9721-z

Kovendan K, Arivoli S, Maheshwaran R, Baskar K, Vincent S. 2012. Larvicidal efficacy of Sphaeranthus indicus, Cleistanthus collinus and Murraya koenigii leaf extracts against filarial vector, Culex quinquefasciatus Say (Diptera: Culicidae). Parasitology Research 111, 1025-1035.

Kumar S, Agrawal D, Patnaik J, Patnaik S. 2012. Analgesic effect of neem (Azadirachta indica) seed oil on albino rats. International Journal of Pharma and Bio Sciences 3, 222-225.

Lokanadhan S, Muthukrishnan P, Jeyaraman S. 2012. Neem products and their agricultural applications. Journal of Biopesticide 5, 72-76.

Liu Y, Liu XC, Liu QY, Niu C, Liu ZL. 2015. Larvicidal activity of Illicium difengpiBN Chang (Schisandraceae) stem bark and its constituent compounds against Aedes aegypti L. Tropical Journal of Pharmaceutical Research 14, 103-109. http://dx.doi.org/10.3390/molecules180910733

Musabyimana T, Saxena RC, Kairu EW, Ogol CPKO, Khan ZR. 2001.Effects of neem seed derivatives on behavioral and physiological responses of the Cosmopolites sordidus (Coleoptera: Curculionidae). Journal of Economic Entomology 94, 449-454.

Murthy JM, Rani PU. 2009. Biological activity of certain botanical extracts as larvicides against the yellow fever mosquito, Aedes aegypti. L. Journal of Biopesticides 2, 72-76.

Mgbemena IC. 2010. Comparative evaluation of larvacidal potentials of three plant extracts on Aedes aegypti.Journal of American Science 6, 435-440.

Mansour SA, Bakr RF, Hamouda LS, Mohamed RI. 2010. Toxic and synergistic properties of several botanical extracts against larval and adult stages of the mosquito, Anopheles pharoensis. Biopesticides International 6, 129-145.

Malwal M, Sarin R. 2011. Antimicrobial efficacy of Murraya koenigii (Linn.) Spreng.root extracts. Indian Journal of Natural Products and Resources2, 48-51.

Maragathavalli S, Brindha S, Kaviyarasi NS, Annadurai B, Gangwar SK. 2012. Mosquitoes larvacidal activity of leaf extract of neem (Azadirachta indica). International Journal of Advanced Biological Research 2, 138-142.

Mazzacano C, Black SH. 2013. Mosquito control methods. Ecologically sound mosquito management in wetlands. An overview of mosquito control practices, the risks, benefits, and nontarget impacts, and recommendations on effective practices that control mosquitoes, reduce pesticide use, and protect wetlands (pp 7-12). Portland. The Xerces Society for Invertebrate Conservation.

Mohan S, Abdelwahab SI, Cheah SC, Sukari MA, Syam S, Shamsuddin N, Mustafa MR. 2013. Apoptosis effect of girinimbine isolated from Murrayakoenigii on lung cancer cells in vitro. Evidence-Based Complementary and Alternative Medicine 2013, 1-12. http://dx.doi.org/10.1155/2013/689865

Parveen M. 2013. The bioactivity of neem (Azadirachta indica A. Juss.) based products against various animal systems. Indian Journal of Applied and Pure Biology 28, 287-289.

Patil P, Patil S, Mane A, Verma S. 2013. Antidiabetic activity of alcoholic extract of neem (Azadirachta indica) root bark. National Journal of Physiology, Pharmacy and Pharmacology 3, 142-146.

Parimi BN, Mopuri R, Meriga B. 2014. The protective effect of Murraya koenigii leaves against carbon tetrachloride- induced hepatic damage in rats. Journal of Costal Life Medicine 2, 313-318. http://dx.doi.org/10.12980/JCLM.2.201414J9

Randhawa MA. 2009. Calculation of LD50 values from the method of Miller and Tainter, 1944.Journal of Ayub Medical College Abbottabad 21, 184-185.

Rageeb MD, Usman MD, Barhate SD. 2012. Phytochemical evaluation and effect of antipyretic activity on Murraya koenigii Spreng. Leaves extract. International Journal of Pharmaceutical and Chemical Sciences 1, 231-236.

Rajendran MP, Pallaiyan BB, Selvaraj N. 2014. Chemical composition, antibacterial and antioxidant profile of essential oil from Murraya koenigii (L.) leaves. Avicenna Journal of Phytomedicine 4, 200-214.

Sun YP, Johnson ER. 1960. Analysis of joint action of insecticide against house flies. Journal of economic entomology 53, 887-892. http://dx.doi.org/10.1093/jee/53.5.887

Shah KJ, Juvekar AR. 2006. Positive inotropic effect of Murraya koenigii (Linn.) spreng extract on an isolated perfused frog heart. Indian Journal of Experimental Biology 44, 481-484.

Sharma US, Sharma UK, Singh A, Sutar N, Singh PJ. 2010. In vitro antihelminth activity of Murraya koenigii Linn. Leaves extracts. International Journal of Pharma and Bio Science 1, 1-4.

Swati D, Pathak A, Burande MD. 2012. Antigenotoxic effect of Murraya koenigii towards cyclophosphamide induced cytogenetic damage in mouse bone marrow cells. International Journal of Pharmacognosy and Phytochemical Research 4, 59-63.

Subramaniam J, Kovendan K, Kumar PM, Murugan K, Walton W. 2012. Mosquito larvacidal activity of Aloe vera (Family: Liliaceae) leaf extract and Bacillus sphaericus, against Chikungunya vector, Aedes aegypti. Saudi Journal of Biological Sciences 19, 503-509. http://dx.doi.org/10.1016/j.sjbs.2012.07.003

Tiwari V, Darmani NA, Yue BY, Shukla D. 2010. In vitro antiviral activity of neem (Azadirachta indica L.) bark extract against herpes simplex virus type-1 infection. Phytotherapy Research 24, 1132-40. http://dx.doi.org/10.1002/ptr.3085

Tembhurne SV, Sakarkar DM. 2012. Anti-obesity and hypoglycemic effect of ethanolic extract of Murraya koenigii (L) leaves in high fatty diet rats. Asian Pacific Journal of Tropical Disease 2, 166-168.

Umesh BT, Leeja L, Thoppil JE. 2014. A study on mosquito larvicidal activity of essential oil of four species of Ocimum against Aedes albopictus. Skuse. International Journal of Institutional Pharmacy and Life Sciences 4, 1-4.

World Health Organization. 2015. Update on the dengue situation in the Western Pacific Region. 1-5 (Assessed on 31/3/2015).

Zaki AHM, Brett J, Ismail E, Azou ML. 2014. Epidemiology of dengue disease in Malaysia (2000-2012): A systemic literature review. PLOS Neglected Tropical Diseases 8, 1-9.