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Research Paper | July 28, 2022

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Detection of virulence genes associated with diarrheagenic pathotypes in Escherichia coli isolates from local beverages and ice samples in Ouagadougou

G. Bsadjo Tchamba, H. Bawa Ibrahim, N. Barro

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Int. J. Biosci.21(1), 192-199, July 2022

DOI: http://dx.doi.org/10.12692/ijb/21.1.192-199

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Abstract

Escherichia coli is one of the foodborne pathogens responsible for diarrhea with approximately 2 million deaths per year. The objective of this study was to determine the virulence genes of diarrheal E. coli isolated from local beverages and ice blocks. One hundred and twenty-one Escherichia coli strains isolated from local beverages and ice blocks were examined for virulence genes associated with diarrheagenic pathotypes. Twenty-one (17.3%) of the 121 E. coli strains carried the virulence genes. Nineteen (5.2%) of the isolates harbored the eae gene. The eae gene present in the EPEC was found in 6 strains isolated from “gnamakoudjii“, 6 strains isolated from “zoom-koom“, 6 strains isolated from ice blocks and 1 strain isolated from “bissap“. Shiga toxin genes, the stx2 gene present in STEC was observed in 2 strains isolated from “zoom-koom” and food ice, respectively. The stx1 and estla genes present in STEC-ETEC were detected only in one strain of E. coli isolated from “zoom-koom“. E. coli produces some virulence factors, which may be involved in human disease, constituting a public health concern.

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Detection of virulence genes associated with diarrheagenic pathotypes in Escherichia coli isolates from local beverages and ice samples in Ouagadougou

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