Search Results for : secondary metabolites

Isolation and characterization of lovastatin producing fungi; investigating the antimicrobial and extracellular enzymatic activities

Lovastatin is the drug used to reduce blood cholesterol serum levels. The present study includes the isolation of new fungal species such as Aspergillus terreus to produce lovastatin and other key secondary metabolites that can be effectively used as antimicrobial agents. New strain was characterized by morphological and molecular identification. Lovastatin was produced by Aspergillus […]

Antimicrobial potential of the crude extracts of an ethno botanically important plant Quisqualis indica Linn.

The organic and inorganic crude extracts of different plant parts of Quisqualis indica Linn; were obtained by maceration technique in n-hexane, chloroform, ethanol and water. The phytochemical screening of the crude extracts disclosed the presence of a variety of secondary metabolites, such as alkaloids, tannins, saponins, terpenoids, flavonoids, cardiac glucosides and reducing sugars. The crude […]

Rapid seed DNA extraction for species identification and diversity analysis of Pumpkin

Cucurbita moschata an economically important species of the family Cucurbitaceae, shows high variability in fruit characteristics. A standardized DNA isolation protocol has been developed from dried pumpkin seeds for polymerase chain reaction for species identification and diversity analysis. Higher concentration of polysaccharides and polyphenols in pumpkin seeds interferes with DNA during its isolation resulting in […]

Chemical composition and bilogical effects of essential oil of Artemisia judaica an endemic plant from central Sahara of Algeria Hoggar

The medicinal plants contain many secondary metabolites that are gifted of different biologic activities as antiparasital, antimicrobial or antioxidant proprieties. Essential oil extracted from dried aerial parts of Artemisia judaica harvested at central Sahara of Algeria Hoggar, was analysed by gas chromatography (GC-FID) and coupled to mass spectrometry (GC-MS).  Thirty one compounds were identified, representing […]

Antioxidant, Antimicrobial and Antileishmanial Study of Different Parts of Peganum harmala

Peganum harmala is bright green, densely foliaged and herbaceous plant, used in folkloric medicine for the treatment of various diseases like asthma, jaundice and lumbago. It contains biologically active secondary metabolites of diverse chemical nature. Therefore present research was designed to determine antioxidant, antimicrobial and antileishmanial potential and phytochemical analysis of different parts of P. […]

Characterization of antibacterial compounds produced by psychrotrophic Alcaligenes faecalis HTP6 isolated from Passu glacier, Pakistan

Low temperature microorganisms can produce secondary metabolites including anticancer, antiviral and antibacterial compounds. The purpose of the study was to evaluate the possibility of using cold adapted bacteria for production of antibiotics. In the present research work, bacteria were isolated from sediment sample, collected from Passu glacier, using R2A medium . These isolates were screened […]

A modified genomic DNA extraction method from leaves of sunflower for PCR based analyzes

Sunflower genomic DNA extraction for PCR (Polymerase Chain Reaction) analyses and similar molecular technics is a challenging issue because of high concentrations of secondary metabolites, i.e. polyphenolics, polysaccharides, tannins and other contaminants. Our modified (Cetyl Trimethyl Ammonium Bromide) CTAB protocol can be performed approximately in two hours and suitable for PCR analyses. It needed small […]

Antimicrobial activity and phytochemical screening of Maerua pseudopetalosa (Glig and Bened.) DeWolf

Chloroform, ethyl acetate and ethanol, which were used for the extraction of Maerua. pseudopetalosa parts, gave nine extracts with different colors, consistencies and extractabilities. Four bacterial strains (Bacillus cereus, Escherichia coli, Staphylococcus aureus and Salmonella typhi, and three fungal species: Aspergillus fumigatus, Aspergillus flavus and Candida albicans) were tested for antimicrobial activities of the extracts. […]