Search Results for : secondary metabolites
Isolation and characterization of lovastatin producing fungi; investigating the antimicrobial and extracellular enzymatic activities
Lovastatin is the drug used to reduce blood cholesterol serum levels. The present study includes the isolation of new fungal species such as Aspergillus terreus to produce lovastatin and other key secondary metabolites that can be effectively used as antimicrobial agents. New strain was characterized by morphological and molecular identification. Lovastatin was produced by Aspergillus […]
Antimicrobial potential of the crude extracts of an ethno botanically important plant Quisqualis indica Linn.
The organic and inorganic crude extracts of different plant parts of Quisqualis indica Linn; were obtained by maceration technique in n-hexane, chloroform, ethanol and water. The phytochemical screening of the crude extracts disclosed the presence of a variety of secondary metabolites, such as alkaloids, tannins, saponins, terpenoids, flavonoids, cardiac glucosides and reducing sugars. The crude […]
Rapid seed DNA extraction for species identification and diversity analysis of Pumpkin
Cucurbita moschata an economically important species of the family Cucurbitaceae, shows high variability in fruit characteristics. A standardized DNA isolation protocol has been developed from dried pumpkin seeds for polymerase chain reaction for species identification and diversity analysis. Higher concentration of polysaccharides and polyphenols in pumpkin seeds interferes with DNA during its isolation resulting in […]
Chemical composition and bilogical effects of essential oil of Artemisia judaica an endemic plant from central Sahara of Algeria Hoggar
The medicinal plants contain many secondary metabolites that are gifted of different biologic activities as antiparasital, antimicrobial or antioxidant proprieties. Essential oil extracted from dried aerial parts of Artemisia judaica harvested at central Sahara of Algeria Hoggar, was analysed by gas chromatography (GC-FID) and coupled to mass spectrometry (GC-MS). Thirty one compounds were identified, representing […]
Antioxidant, Antimicrobial and Antileishmanial Study of Different Parts of Peganum harmala
Peganum harmala is bright green, densely foliaged and herbaceous plant, used in folkloric medicine for the treatment of various diseases like asthma, jaundice and lumbago. It contains biologically active secondary metabolites of diverse chemical nature. Therefore present research was designed to determine antioxidant, antimicrobial and antileishmanial potential and phytochemical analysis of different parts of P. […]
Characterization of antibacterial compounds produced by psychrotrophic Alcaligenes faecalis HTP6 isolated from Passu glacier, Pakistan
Low temperature microorganisms can produce secondary metabolites including anticancer, antiviral and antibacterial compounds. The purpose of the study was to evaluate the possibility of using cold adapted bacteria for production of antibiotics. In the present research work, bacteria were isolated from sediment sample, collected from Passu glacier, using R2A medium . These isolates were screened […]
Gibberellin and indole acetic acid production capacity of endophytic fungi isolated from ZEA MAYS L.
Endophytic fungi are endosymbionts of majority of plants and having role in plant growth promotion by producing important secondary metabolites such as gibberellin and auxin. A total of 29 different endophytic fungal strains were isolated from the roots (21) and leaves (8) of Zea mays L. and screened for GA and IAA producing capacity. Out […]
A modified genomic DNA extraction method from leaves of sunflower for PCR based analyzes
Sunflower genomic DNA extraction for PCR (Polymerase Chain Reaction) analyses and similar molecular technics is a challenging issue because of high concentrations of secondary metabolites, i.e. polyphenolics, polysaccharides, tannins and other contaminants. Our modified (Cetyl Trimethyl Ammonium Bromide) CTAB protocol can be performed approximately in two hours and suitable for PCR analyses. It needed small […]
Cloning and sequence analysis of a flavanone 3-hydroxylase gene from Prunus persica (L.) Batsch
Anthocyanins as a type of plant flavonoids, is a kind of important plant secondary metabolites, which is catalyzed by a series of enzymes such as flavanone 3-hydroxylase (F3H). Here a F3H gene, designated as PpF3H, was cloned from Prunus persica (L.) Batsch. The full-length cDNA of PpF3H gene was 1453 bp in length, containing a […]
Antimicrobial activity and phytochemical screening of Maerua pseudopetalosa (Glig and Bened.) DeWolf
Chloroform, ethyl acetate and ethanol, which were used for the extraction of Maerua. pseudopetalosa parts, gave nine extracts with different colors, consistencies and extractabilities. Four bacterial strains (Bacillus cereus, Escherichia coli, Staphylococcus aureus and Salmonella typhi, and three fungal species: Aspergillus fumigatus, Aspergillus flavus and Candida albicans) were tested for antimicrobial activities of the extracts. […]