Antioxidant, Antimicrobial and Antileishmanial Study of Different Parts of Peganum harmala

Peganum harmala is bright green, densely foliaged and herbaceous plant, used in folkloric medicine for the treatment of various diseases like asthma, jaundice and lumbago. It contains biologically active secondary metabolites of diverse chemical nature. Therefore present research was designed to determine antioxidant, antimicrobial and antileishmanial potential and phytochemical analysis of different parts of P. […]

Characterization of antibacterial compounds produced by psychrotrophic Alcaligenes faecalis HTP6 isolated from Passu glacier, Pakistan

Low temperature microorganisms can produce secondary metabolites including anticancer, antiviral and antibacterial compounds. The purpose of the study was to evaluate the possibility of using cold adapted bacteria for production of antibiotics. In the present research work, bacteria were isolated from sediment sample, collected from Passu glacier, using R2A medium . These isolates were screened […]

Gibberellin and indole acetic acid production capacity of endophytic fungi isolated from ZEA MAYS L.

Endophytic fungi are endosymbionts of majority of plants and having role in plant growth promotion by producing important secondary metabolites such as gibberellin and auxin. A total of 29 different endophytic fungal strains were isolated from the roots (21) and leaves (8) of Zea mays L. and screened for GA and IAA producing capacity. Out […]

A modified genomic DNA extraction method from leaves of sunflower for PCR based analyzes

Sunflower genomic DNA extraction for PCR (Polymerase Chain Reaction) analyses and similar molecular technics is a challenging issue because of high concentrations of secondary metabolites, i.e. polyphenolics, polysaccharides, tannins and other contaminants. Our modified (Cetyl Trimethyl Ammonium Bromide) CTAB protocol can be performed approximately in two hours and suitable for PCR analyses. It needed small […]

Cloning and sequence analysis of a flavanone 3-hydroxylase gene from Prunus persica (L.) Batsch

Anthocyanins as a type of plant flavonoids, is a kind of important plant secondary metabolites, which is catalyzed by a series of enzymes such as flavanone 3-hydroxylase (F3H). Here a F3H gene, designated as PpF3H, was cloned from Prunus persica (L.) Batsch. The full-length cDNA of PpF3H gene was 1453 bp in length, containing a […]

Antimicrobial activity and phytochemical screening of Maerua pseudopetalosa (Glig and Bened.) DeWolf

Chloroform, ethyl acetate and ethanol, which were used for the extraction of Maerua. pseudopetalosa parts, gave nine extracts with different colors, consistencies and extractabilities. Four bacterial strains (Bacillus cereus, Escherichia coli, Staphylococcus aureus and Salmonella typhi, and three fungal species: Aspergillus fumigatus, Aspergillus flavus and Candida albicans) were tested for antimicrobial activities of the extracts. […]

Antifungal activity from aerial part extracts of Leptadeniapyrotechnica (Forsskal.) Decne from the Cholistan Desert, Pakistan

This study was carried out with an objective to investigate the antifungal potentials of aerial part of leptadeniapyrotechnica (Forssk) Decne. The aim of the study is to assess the antifungal activity and to determine the zone of inhibition of extracts on fungal strains. The experiment based on ethno-medicinal survey, in the present study, the antifungal […]

Increasing of Chavicol o-methyl transferase gene expression (CVOMT) and methyl Chavicol value of basil (Ocimum basilicum) by salicylic acid

Basil (Ocimum basilicum), is a medicinal plant mint family (Lamiaceae) which contains cyclic compounds and essential oil, and it has antibacterial and antioxidant properties. From Long ago, Basil has been used for treatment of headache, cough, diarrhea and fever, inflammation of the throat and stomach pain in Iran. Basil essential oil is mainly containing phenylpropanoids […]

Genetic diversity of Iranian indigenous fennel (Foeniculum vulgare Mill.) populations using agronomic traits and essential oil

Fennel (Foeniculum vulgare Mill.) is one of the most important medicinal plants. It has a variety of secondary metabolites which are used in treatment of heart, nerves, digestive disorders and particularly alzheimer’s disease. In this research, the genetic diversity of fifteen Iranian populations and four foreign populations of fennel from Germany and Turkey were evaluated […]

Investigation of anti proliferative properties and antioxidant activity of aerial parts ethanolic extract of Hypericum perforatum L. by breast cancer 4T1 cell lines

The traditional use of herbal medicine has increased significantly in these recent years. Nowadays it is very desirable to investigate and discover new antibacterial and anticancer agents from natural products and medicinal plants. The natural products obtained from organisms such as medicinal plants (secondary metabolites) are known as a powerful source to supplement therapy and […]