In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir
Paper Details
In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir
Abstract
The aim of this work is to determine the quantity, the quality, the antimicrobial activity and the antioxidant power of various extracts of the flavonoids obtained from the flowers of Tamarix africana Poir. The quantification of the extracts obtained was revealed in high yield of the flavonoids with respectively: the methanoic extracts (26.31%), the extracts of the aqueous phase (19.29%), the extracts of ethyl acetate (0.87%), the extracts of petroleum ether (0.18%). The qualitative study, using the thin-layer chromatography (TLC), showed the dominance of Flavonols, flavones, isoflavones, flavanones and 3-glycosidic Anthocyanidins. The study of microbial activity revealed an important bactericidal power for the extracts of the aqueous phase on Gram + bacteria with a disc of inhibition of 24±1mm on Staphylococcus aureus ATCC43300 and 20±1mm on Staphylococcus aureus ATCC 25923, For the antifungal activity all the extracts gave important effects on Podosphaera leucotrichia (apple powdery mildew), with a maximum disc inhibition of 20±1mm for the ethyl acetate extracts, on the other hand alone The ether extracts of the petrol which showed an inhibitory effect on Penicillium sp. The antioxidant study, expressed as a percentage of DPPH, showed a high efficiency of the various extracts; In particular that of the ethyl acetate extract which inhibits oxidation and traps the free radicals at 100%, which demonstrating the use of this plant in traditional medicine for the treatment of certain types of cancer.
Athamena S. 2009. Etude Quantitative Des Flavonoides Des Graines De Cuminum Cyminum Et Les Feuilles De Rosmarinus officinalis Et L’evaluation De L’activite Biologique. Biochimie Appliquée 7, 126-133
Ben Mansour A, Porter EA, Kite GC, Simmonds MS, Abdelhedi R, Bouaziz M. 2015. Phenolic profile characterization of Chemlali olive stones by liquid chromatography-ion trap mass spectrometry. J Agric Food Chem 63, 1990-1995.
Benabdallah A, Rahmoune C, Boumendjel M, Aissi O, Messaoud C. 2016. Total phenolic content and antioxidant activity of six wild Mentha species (Lamiaceae) from Northeast of Algeria. Asian Pac J Trop Biomed 6, 760-766.
Biswas N, Saha S, Ali MK. 2014. Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. Asian Pac J Trop Biomed 4, 792-798.
Bonina F, Lanza M, Montenegro L, Puglisi C, Tomaino A, Trombetta D, Castelli F, Saija A. 1996. Flavonoids as potential protective agents against photo-oxidative skin damage. International Journal of Pharmaceutics 145, 87-94.
Celiktas OY, Hames Kocabas, EE, Bedir, E, Vardar Sukan F, Ozek T, Baser KHC. 2007. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chem 100, 553-559.
Huang G, Jiang J, Dai D. 2008. Antioxidative and antibacterial activity of the methanol extract of Artemisia anomala S. Moore. African Journal of Biotechnol. 7, 1335-1338.
Ishtiaq S, Ahmad M, Hanif U, Akbar S, Mehjabeen, Kamran SH. 2014. Phytochemical and in vitro antioxidant evaluation of different fractions of Amaranthus graecizans subsp. silvestris (Vill.) Brenan. Asian Pac J Trop Med 7, 342-347.
Khabtane A, Rahmoune C. 2010. Contribution à de l’étude de l’effet du biotope sur la richesse floristique et variabilité morphologique chez le Tamarix sp. dans les zones aride de la région de khenchela, thèse du Magister, Université de Constantine, Algérie181-190.
Khabtane A, Rahmoune C. 2012. Effet du biotope sur la diversité floristique et le polymorphisme phénotypique des groupements à Tamarix africana Poir. dans les zones arides de la région de Khenchele (Est Algérien), Journal of Agriculture and Environnent for International Development 106, 123-137.
Ksouri R, Falleh H, Megdiche W, Trabelsi N, Mhamdi B, Chaieb K, Bakrouf A, hristian Magné C, Abdelly C. 2009. Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L. and related polyphenolic constituents. Food and Chemical Toxicology 47, 2083-2091.
Marfak A. 2003. Radiolyse gamma des flavonoïdes. Etude de Leur reactivite avec les radicaux issus des Alcools: formation de depsides. Thèse de doctorat, Université Limoges, Belgique 225-230.
Markowicz Bastos DH, Saldanha LA, Catharino RR, Sawaya ACHF, Cunha IBS, Carvalho PO, Eberlin MN. 2007. Phenolic Antioxidants Identified by ESI-MS from Yerba Maté (Ilex paraguariensis) and Green Tea (Camelia sinensis) Extracts. Molecules 12, 423-432.
Parmar VS, Bisht KS, Sharma SK, Jain R, Taneja P, Singh S, Simonsen O, Boll PM. 1994. Highly oxygenated bioactive flavones from Tamarix. Phytochemistry 36, 507-511.
Rasooli I, Fakoor MH, Yadegarinia D, Gachkar L, Allameh A, Rezaei MB. 2008. Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils. International J of Food Microbiology 122, 135-139.
Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R. 2005. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem 91, 621-632.
Saıdana D, Mahjoub MA, Boussaada O, Chriaa J, Cheraif I, Daami M, Mighri Z, Helal AN. 2008. Chemical composition and antimicrobial activity of volatile compounds of Tamarix boveana (Tamaricaceae). Microbiological Research 163, 445-455.
Wang B, Ren S, Li G, Guan H. 2009. Studies on antitumor steroids and flavonoids from Tamarix chinensis Lour. Chinese Pharmacetical Journal 44, 576-580.
Abdelhamid Khabtane, Azzeddine Zeraib, Laiche Aouidane, Wahiba Kara Ali, Fatima Zohra Belguidoum, Chabane Rahmoune (2017), In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir; IJB, V11, N1, July, P417-426
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